TNF-alpha Protein, Human, Recombinant

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TNF-alpha Protein, Human, Recombinant: 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
Please contact us for more information.
活性
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is typically 5-50 pg/mL.
タンパク質の構築
A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
NP No.
発現ホスト
E. coli
Human
予測N末端
Met
分子量
The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.4 kDa which is also estimated by SDS-PAGE.
バッファー
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TNF-alpha Protein, Human, Recombinant: 画像

TNF-alpha Protein, Human, Recombinant: 別名

DIF Protein, Human; TNF-alpha Protein, Human; TNFA Protein, Human; TNFSF2 Protein, Human

TNF-alpha 背景情報

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD12a; p55/6) and TNF-R2 (TNF receptor type 2; CD12b; p75/8), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
完全な名称
tumor necrosis factor
研究分野
参考文献
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • Houttuynia cordata blocks HSV infection through inhibition of NF-κB activation
    Author
    Chen, X;Wang, Z;Yang, Z;Wang, J;Xu, Y;Tan, RX;Li, E;
    Year
    2011
    Journal
    Antiviral Res.
    Application
    cell-based (culture)
  • Inhibition of herpes simplex virus infection by oligomeric stilbenoids through ROS generation
    Author
    Chen, X;Qiao, H;Liu, T;Yang, Z;Xu, L;Xu, Y;Ge, HM;Tan, RX;Li, E;
    Year
    2012
    Journal
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    Application
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    Appl. Microbiol. Biotechnol.
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    Chen, X;Wang, C;Xu, L;Chen, X;Wang, W;Yang, G;Tan, RX;Li, E;Jin, Y;
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    2013
    Journal
    Evid Based Complement Alternat Med
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    Year
    2015
    Journal
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  • CYLD Promotes TNF-α-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells
    Author
    Lin, X;Chen, Q;Huang, C;Xu, X;
    Year
    2016
    Journal
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    Application
    cell-based
  • Neutralization of TNFα in tumor with a novel nanobody potentiates paclitaxel-therapy and inhibits metastasis in breast cancer
    Author
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    Author
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    Year
    2017
    Journal
    PLoS ONE
    Application
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    Author
    Du, J;Rehm, BHA;
    Year
    2017
    Journal
    Microb. Cell Fact.
    Application
    Elisa;target protein
  • T0001, a variant of TNFR2-Fc fusion protein, exhibits improved Fc effector functions through increased binding to membrane-bound TNFα
    Author
    Shen, Y;Li, G;Gu, C;Chen, B;Chen, A;Li, H;Gao, B;Liang, C;Wu, J;Yang, T;Jin, L;Su, Y;
    Year
    2017
    Journal
    PLoS ONE
    Application
    FACS
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    Author
    Zhang, C;Jiang, H;Wang, P;Liu, H;Sun, X;
    Year
    2017
    Journal
    Sci Rep
    Application
    cell-based
  • TGF-α Overexpression in Breast Cancer Bone Metastasis and Primary Lesions and TGF-α Enhancement of Expression of Procancer Metastasis Cytokines in Bone Marrow Mesenchymal Stem Cells
    Author
    Sun, J;Cui, H;Gao, Y;Pan, Y;Zhou, K;Huang, J;Lan, J;Wei, Q;Liu, X;Liu, L;Xian, C;
    Year
    2018
    Journal
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    Application
    cell-based
  • Downregulation of tumor suppressor RACK1 by Helicobacter pylori infection promotes gastric carcinogenesis through the integrin β-1/NF-κB signaling pathway
    Author
    Hu, Y;Liu, J;Li, X;Cai, Y;He, C;Li, N;Xie, C;Xiong, Z;Ge, Z;Lu, N;Zhu, Y;
    Year
    2019
    Journal
    Cancer Letters
    Application
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