Goat Anti-Mouse IgG-Fc Secondary Antibody (HRP)

 
 
Overview
 

Description:

Goat polyclonal Secondary Antibody to Mouse IgG-Fc (HRP).

Host

Goat

Target species:

Mouse IgG-Fc

Specificity:

By ELISA this antibody reacts specifically with Mouse IgG-Fc.

 

Has cross-reactivity with rat IgG(5.4%)

Minimal cross-reactivity with human IgG, rabbit IgG, bovine IgG, horse IgG, guinea pig IgG

Applications:

WB, ELISA, IHC-P, IP

 
Properties

Form:

Liquid

Storage & Stability:

Protected from prolonged exposure to light. Store at +4℃ short term (1-2 weeks) (Preservative is recommended to avoid contamination). Upon delivery aliquot. Store at -20℃ to -80℃ for twelve months from date of receipt. Avoid repeated freeze-thaw cycles.

Constituents:

PBS

Purification notes:

Immunogen affinity purified

Formulation:

Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

 
Applications

The application notes include recommended starting dilutions, optimal dilutions/concentrations should be determined by the end user.

 

IHC-P

0.5-10 μg/mL

ELISA

0.01-0.5 μg/mL

WB

0.05-1 μg/mL (Colormetric: 0.1-1 μg/mL; Chemiluminescent: 0.05-1 μg/mL)

 IP

0.05-1 μg/mL

 
  • A universal and enzyme-free immunoassay platform for biomarker detection based on gold nanoparticle enumeration with a dark-field microscope
    Author
    Wu, X;Li, T;Tao, G;Lin, R;Pei, X;Liu, F;Li, N;
    Year
    2017
    Journal
    Analyst
    Application
    Preparation of secondary antibody–AuNPs
  • A universal and enzyme-free immunoassay platform for biomarker detection based on gold nanoparticle enumeration with dark-field microscope
    Author
    Wu, X;Li, T;Tao, G;Lin, R;Pei, X;Liu, F;Li, N;
    Year
    2017
    Journal
    Analyst
    Application
    immunoassay
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