VISTA Antibody Guideline: Preparation, Dilution and Applications

VISTA Antibody Overview

There are 9 VISTA antibodies which are validated in multiple tissues with various applications, including FCM, IHC-P, ELISA, ICC/IF. There are 1 VISTA antibody for FCM, 3 VISTA antibody for IHC-P, 6 VISTA antibody for ELISA, 2 VISTA antibody for ICC/IF. Among all these VISTA antibodies, there are 2 anti-VISTA mouse monoclonal antibodies , 7 anti-VISTA rabbit polyclonal antibodies . All the VISTA anbodies are produced in house and all are in stock. VISTA antibody customerized service is available.


VISTA antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human B7-H5 / GI24 / VISTA (rh C10ORF54; Catalog#13482-H08H; AAH20568.1; Met1-Ala194). Total IgG was purified by Protein A affinity chromatography.

VISTA antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000

VISTA Antibody immunohistochemistry

VISTA Antibody immunohistochemistry Preparation

Produced in rabbits immunized with purified, recombinant Human VISTA/GI24/B7-H5 (rh VISTA/GI24/B7-H5; Catalog#13482-H08H; AAH20568.1; Met1-Ala194). VISTA/GI24/B7-H5 specific IgG was purified by Human VISTA/GI24/B7-H5 affinity chromatography.

VISTA Antibody immunohistochemistry Validated

Application IHC
Dilution 1:500-1:2000