RSPO1 Protein Overview: Sequence, Structure, Function and Protein Interaction

RSPO1 Protein Sequence

Species Human RSPO1 protein
Length 263
Mass (Da) 28959
Sequence Human RSPO1 protein sequence
Species Mouse RSPO1 protein
Length 265
Mass (Da) 29332
Sequence Mouse RSPO1 protein sequence
Species
Length
Mass (Da)
Sequence

RSPO1 Protein Molecular Weight & PI

R-spondin-1 precursor (Roof plate-specific spondin-1) (hRspo1) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 21-263 R-spondin-1.

Molecular weight (Da)

26764.51

Theoretical pI

9.44

RSPO1 Protein Structure

Structure of the ectodomain of LGR5 in complex with R-spondin-1 (Fu1Fu2) in P21 crystal form
Deposited
2013-06-11   Released:  2013-06-19
Deposition Author(s)
Peng, W.C., de Lau, W., Forneris, F., Granneman, J.C.M., Huch, M., Clevers, H., Gros, P.
Organism(s)
Homo sapiens
Expression System
Homo sapiens
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
3.2000 Å
R-Value Free
0.290
R-Value Work
0.246
4BSS From PDB

Human RSPO1 protein Secondary structure

RSPO1 Protein Interaction

Recombinant RSPO1 Protein Feature

RSPO1 Protein, Human, Recombinant

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 20-120 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.

Recombinant RSPO1 protein citations

Title
mTORC1 in the Paneth cell niche couples intestinal stem-cell function to calorie intake
Year
2012
Author
ÖH Yilmaz, P Katajisto, DW Lamming, Y Gültekin…
Journal
Nature
Title
R-Spondin potentiates Wnt/β-catenin signaling through orphan receptors LGR4 and LGR5
Year
2012
Author
Ruffner, H;Sprunger, J;Charlat, O;Leighton-Davies, J;Grosshans, B;Salathe, A;Zietzling, S;Beck, V;Therier, M;Isken, A;Xie, Y;Zhang, Y;Hao, H;Shi, X;Liu, D;Song, Q;Clay, I;Hintzen, G;Tchorz, J;Bouchez, LC;Michaud, G;Finan, P;Myer, VE;Bouwmeester, T;Porter, J;Hild, M;Bassilana, F;Parker, CN;Cong, F;
Journal
PLoS ONE
Title
Cables1 is a tumor suppressor gene that regulates intestinal tumor progression in ApcMin mice
Year
2013
Author
LR Zukerberg
Journal
Cancer biology & therapy
Title
Lgr4/Gpr48 negatively regulates TLR2/4-associated pattern recognition and innate immunity by targeting CD14 expression
Year
2013
Author
Du, B;Luo, W;Li, R;Tan, B;Han, H;Lu, X;Li, D;Qian, M;Zhang, D;Zhao, Y;Liu, M;
Journal
J. Biol. Chem.
Title
Lgr4-mediated Wnt/β-catenin signaling in peritubular myoid cells is essential for spermatogenesis
Year
2013
Author
Qian, Y;Liu, S;Guan, Y;Pan, H;Guan, X;Qiu, Z;Li, L;Gao, N;Zhao, Y;Li, X;Lu, Y;Liu, M;Li, D;
Journal
Development
Title
Differential effects of 2- and 3-series E-prostaglandins on in vitro expansion of Lgr5+ colonic stem cells
Year
2014
Author
Fan, YY;Davidson, LA;Callaway, ES;Goldsby, JS;Chapkin, RS;
Journal
Carcinogenesis
Title
Luminal Microbes Promote Monocyte–Stem Cell Interactions Across a Healthy Colonic Epithelium
Year
2014
Author
DA Skoczek, P Walczysko, N Horn…
Journal
The Journal of Immunology
Title
Microbiome-derived Tryptophan Metabolites and Their Aryl Hydrocarbon Receptor-Dependent Agonist and Antagonist Activities
Year
2014
Author
Jin, UH;Lee, SO;Sridharan, G;Lee, K;Davidson, LA;Jayaraman, A;Chapkin, R;Alaniz, R;Safe, SH;
Journal
Mol. Pharmacol.
Title
YAP/TAZ incorporation in the β-catenin destruction complex orchestrates the Wnt response
Year
2014
Author
Azzolin, L;Panciera, T;Soligo, S;Enzo, E;Bicciato, S;Dupont, S;Bresolin, S;Frasson, C;Basso, G;Guzzardo, V;Fassina, A;Cordenonsi, M;Piccolo, S;
Journal
Cell
Title
Suppression of intestinal tumorigenesis in Apc mutant mice upon Musashi-1 deletion
Year
2017
Author
Wolfe, AR;Ernlund, A;McGuinness, W;Lehmann, C;Carl, K;Balmaceda, N;Neufeld, KL;
Journal
J. Cell. Sci
Title
Critical intestinal cells originate from the host in enteroid-derived tissue-engineered intestine
Year
2018
Author
Cromeens, BP;Wang, Y;Liu, Y;Johnson, J;Besner, GE;
Journal
Journal of Surgical Research
Title
Fasting Activates Fatty Acid Oxidation to Enhance Intestinal Stem Cell Function during Homeostasis and Aging
Year
2018
Author
Mihaylova, MM;Cheng, CW;Cao, AQ;Tripathi, S;Mana, MD;Bauer-Rowe, KE;Abu-Remaileh, M;Clavain, L;Erdemir, A;Lewis, CA;Freinkman, E;Dickey, AS;La Spada, AR;Huang, Y;Bell, GW;Deshpande, V;Carmeliet, P;Katajisto, P;Sabatini, DM;Yilmaz, ÖH;
Journal
Cell Stem Cell
Title
Ketone Body Signaling Mediates Intestinal Stem Cell Homeostasis and Adaptation to Diet
Year
2019
Author
Cheng, CW;Biton, M;Haber, AL;Gunduz, N;Eng, G;Gaynor, LT;Tripathi, S;Calibasi-Kocal, G;Rickelt, S;Butty, VL;Moreno-Serrano, M;Iqbal, AM;Bauer-Rowe, KE;Imada, S;Ulutas, MS;Mylonas, C;Whary, MT;Levine, SS;Basbinar, Y;Hynes, RO;Mino-Kenudson, M;Deshpande, V;Boyer, LA;Fox, JG;Terranova, C;Rai, K;Piwnica-Worms, H;Mihaylova, MM;Regev, A;Yilmaz, ÖH;
Journal
Cell

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