p38 Antibody Guideline: Preparation, Dilution and Applications

p38 Antibody Overview

There are 4 p38 antibodies which are validated in multiple tissues with various applications, including WB, ELISA, IHC-P, ICC/IF, IP. There are 3 p38 antibody for WB, 4 p38 antibody for ELISA, 2 p38 antibody for IHC-P, 2 p38 antibody for ICC/IF, 2 p38 antibody for IP. Among all these p38 antibodies, there are 1 anti-p38 mouse monoclonal antibodies , 3 anti-p38 rabbit polyclonal antibodies . All the p38 anbodies are produced in house and all are in stock. p38 antibody customerized service is available.

p38 gene family antibody

Approved symbol Common name
MAPK1 ERK2 antibody
MAPK13 P38 Delta/MAPK13 antibody
MAPK9 JNK2 antibody
Approved symbol Common name
MAPK12 P38 Gamma/MAPK12 antibody
MAPK8 JNK1 antibody

p38 Antibody ELISA

p38 antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human p38 alpha / MAPK14 (rh MAPK14; Catalog#10646-H07B; NP_001306.1; Met1-Ser360). Total IgG was purified by Protein A affinity chromatography.

p38 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

p38 Antibody western blot

p38 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human p38 alpha / MAPK14 (rh p38 alpha / MAPK14; Catalog#10081-H07B; NP_620581.1; Met1-Ser360). p38 alpha / MAPK14 specific IgG was purified by Human p38 alpha / MAPK14 affinity chromatography.

p38 Antibody western blot Validated

Application WB
Dilution 1:500-1:2000
Notes  

p38 Antibody immunohistochemistry

p38 Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human p38 alpha/MAPK14 (rh p38 alpha/MAPK14; Catalog#10081-H07B; NP_620581.1; Met1-Ser360). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

p38 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

p38 Antibody immunoprecipitation

p38 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human p38 alpha / MAPK14 (rh p38 alpha / MAPK14; Catalog#10081-H07B; NP_620581.1; Met1-Ser360). p38 alpha / MAPK14 specific IgG was purified by Human p38 alpha / MAPK14 affinity chromatography.

p38 Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

p38 Antibody ICC / IF

p38 Antibody ICC / IF Preparation

Produced in rabbits immunized with purified, recombinant Human p38 alpha/MAPK14 (rh p38 alpha/MAPK14; Catalog#10081-H07B; NP_620581.1; Met1-Ser360). p38 alpha/MAPK14 specific IgG was purified by Human p38 alpha/MAPK14 affinity chromatography.

p38 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:1500-1:50000
Notes