MMP-2 Protein Overview: Sequence, Structure, Function and Protein Interaction

MMP-2 Protein Overview

MMP-2 reagents

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD (Nagase et al., 1992). Type IV collagenase is a metalloproteinase that specifically cleaves type IV collagen, the major structural component of basement membranes (120090, 120130). The metastatic potential of tumor cells has been found to correlate with the activity of this enzyme.

MMP-2 protein family

Belongs to the peptidase M10A family.

MMP-2 protein name

Recommended name
72 kDa type IV collagenase
Short name
MMP-2
Aliases
TBE-1
Alternative name
72 kDa gelatinase Gelatinase A Matrix metalloproteinase-2 TBE-1 PEX

MMP-2 Protein Sequence

Species Human MMP-2 protein
Length 660
Mass (Da) 73882
Sequence Human MMP-2 protein sequence
Species Mouse MMP-2 protein
Length 662
Mass (Da) 74102
Sequence Mouse MMP-2 protein sequence
Species Rat MMP-2 protein
Length 662
Mass (Da) 74149
Sequence Rat MMP-2 protein sequence

MMP-2 Protein Molecular Weight & PI

72 kDa type IV collagenase precursor (EC 3.4.24.24) (72 kDa gelatinase) (Gelatinase A) (Matrix metalloproteinase-2) (MMP-2) (TBE-1) [Contains: PEX] Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 110-660 72 kDa type IV collagenase.

Molecular weight (Da)

62059.41

Theoretical pI

5.02

MMP-2 Protein Structure

Catalytic domain of proMMP-2 E404Q mutant
Deposited
2001-07-12   Released:  2002-08-22
Deposition Author(s)
Bergmann, U., Tuuttila, A., Tryggvason, K., Morgunova, E.
Organism(s)
Homo sapiens
Expression System
Trichoplusia ni
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.6600 Å
R-Value Free
0.303
R-Value Work
0.271
1EAK From PDB

Human MMP-2 protein Secondary structure

MMP-2 Protein Interaction

Recombinant MMP-2 Protein Feature

MMP-2 Protein, Human, Recombinant

High Purity
> 90 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
1. Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog # 27076). The specific activity is > 1,000 pmoles/min/µg. 2. Measured by its binding ability in a functional ELISA. 3. Immobilized human MMP2 at 10 μg/mL (100 μl/well) can bind human TIMP2/Fc (Cat: 10396-H01H). The EC50 of human TIMP2/Fc is 0.02 μg/mL. (Activation description: The proenzyme needs to be activated by APMA for an activated form)

Recombinant MMP-2 protein citations

Title
Upconversion fluorescence resonance energy transfer based biosensor for ultrasensitive detection of matrix metalloproteinase-2 in blood
Year
2012
Author
Y Wang, P Shen, C Li, Y Wang, Z Liu
Journal
Analytical chemistry
Title
A graphene oxide-based FRET sensor for rapid and sensitive detection of matrix metalloproteinase 2 in human serum sample
Year
2013
Author
Song, E;Cheng, D;Song, Y;Jiang, M;Yu, J;Wang, Y;
Journal
Biosens Bioelectron
Title
Enzyme-responsive liposomes modified adenoviral vectors for enhanced tumor cell transduction and reduced immunogenicity
Year
2013
Author
Wan, Y;Han, J;Fan, G;Zhang, Z;Gong, T;Sun, X;
Journal
Biomaterials
Title
Preparation and assembly of collagen–DNA complex on an electrode surface and its application to protein analysis
Year
2013
Author
Ning, L;Miao, P;Gao, T;Wang, H;Li, G;
Journal
Electrochimica Acta
Title
Fiber-optic protease sensor based on the degradation of thin gelatin films
Year
2014
Author
Schyrr, B;Boder-Pasche, S;Ischer, R;Smajda, R;
Journal
Sensing and Bio-Sensing Research
Title
Signal on fluorescence biosensor for MMP-2 based on FRET between semiconducting polymer dots and a metal organic framework
Year
2014
Author
Yang, W;Zhang, G;Weng, W;Qiu, B;Guo, L;Lin, Z;
Journal
RSC Advances
Title
Bio-inspired polymer envelopes around adenoviral vectors to reduce immunogenicity and improve in vivo kinetics
Year
2015
Author
Fan, G;Fan, M;Wang, Q;Jiang, J;Wan, Y;Gong, T;Zhang, Z;Sun, X;
Journal
Acta Biomater
Title
Modular Design and Facile Synthesis of Enzyme-Responsive Peptide-Linked Block Copolymers for Efficient Delivery of Doxorubicin
Year
2016
Author
Ke, W;Li, J;Zhao, K;Zha, Z;Han, Y;Wang, Y;Yin, W;Zhang, P;Ge, Z;
Journal
Biomacromolecules
Title
A Peptide Cleavage-Based Ultrasensitive Electrochemical Biosensor with an Ingenious Two-Stage DNA Template for Highly Efficient DNA Exponential Amplification
Year
2017
Author
Wang, D;Chai, Y;Yuan, Y;Yuan, R;
Journal
Anal. Chem.
Title
A tumour microenvironment-responsive polymeric complex for targeted depletion of tumour-associated macrophages (TAMs)
Year
2017
Author
Wang, Y;Guo, G;Feng, Y;Long, H;Ma, D;Leung, C;Dong, L;Wang, C;
Journal
Journal of Materials Chemistry B
Title
Activatable Near-Infrared Probe for Fluorescence Imaging of γ-Glutamyl Transpeptidase in Tumor Cells and In Vivo
Year
2017
Author
Luo, Z;Feng, L;An, R;Duan, G;Yan, R;Shi, H;He, J;Zhou, Z;Ji, C;Chen, HY;Ye, D;
Journal
Chemistry
Title
Matrix Metalloproteinase-Responsive Multifunctional Peptide-Linked Amphiphilic Block Copolymers for Intelligent Systemic Anticancer Drug Delivery
Year
2017
Author
Ke, W;Zha, Z;Mukerabigwi, JF;Chen, W;Wang, Y;He, C;Ge, Z;
Journal
Bioconjug. Chem.
Title
Peptide Microarray-Based Metal Enhanced Fluorescence Assay for Multiple Profiling of Matrix Metalloproteinases Activities
Year
2017
Author
Lei, Z;Zhang, H;Wang, Y;Meng, X;Wang, Z;
Journal
Anal. Chem.
Title
PtNPs as Scaffolds to Regulate Interenzyme Distance for Construction of Efficient Enzyme Cascade Amplification for Ultrasensitive Electrochemical Detection of MMP-2
Year
2017
Author
Kou, B;Chai, Y;Yuan, Y;Yuan, R;
Journal
Anal. Chem.
Title
Smart Asymmetric Vesicles with Triggered Availability of Inner Cell-Penetrating Shells for Specific Intracellular Drug Delivery
Year
2017
Author
Li, J;Xiao, S;Xu, Y;Zuo, S;Zha, Z;Ke, W;He, C;Ge, Z;
Journal
ACS Appl Mater Interfaces
Title
Evaluation of Matrix Metalloproteinase Inhibition by Peptide Microarray-Based Fluorescence Assay on Polymer Brush Substrate and in Vivo Assessment
Year
2017
Author
Lei, Z;Chen, H;Zhang, H;Wang, Y;Meng, X;Wang, Z;
Journal
ACS Appl Mater Interfaces
Title
Dual-reaction triggered sensitivity amplification for ultrasensitive peptide-cleavage based electrochemical detection of matrix metalloproteinase-7
Year
2018
Author
Zheng, Y;Ma, Z;
Journal
Biosensors and Bioelectronics
Title
A coiled-coil masking domain for selective activation of therapeutic antibodies
Year
2019
Author
Trang, VH;Zhang, X;Yumul, RC;Zeng, W;Stone, IJ;Wo, SW;Dominguez, MM;Cochran, JH;Simmons, JK;Ryan, MC;Lyon, RP;Senter, PD;Levengood, MR;
Journal
Nat. Biotechnol.
Title
Peptide Microarray-Based Fluorescence Assay for Quantitatively Monitoring the Tumor-Associated Matrix Metalloproteinase-2 Activity
Year
2019
Author
Jian, M;Su, M;Gao, J;Wang, Z;
Journal
Sensors and Actuators B: Chemical

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