ICOS ligand Protein Overview: Sequence, Structure, Function and Protein Interaction

ICOS ligand Protein Overview

The costimulatory B7 molecules (e.g., B7-1, or CD80; 112203) signal through CD28 (186760) family molecules, such as CD28, CTLA4 (123890), and ICOS (604558). By searching sequence databases for human homologs of the mouse B7-like gene Gl50, Ling et al. (2000) identified a brain cDNA, KIAA0653 (Ishikawa et al., 1998), containing similar as well as unrelated sequences. Using 3-prime RACE analysis of peripheral blood leukocytes (PBLs) and RT-PCR of leukocyte RNA, Ling et al. (2000) isolated a cDNA encoding a 309-amino acid human B7 homolog, which they termed GL50, that is 42% identical to the mouse sequence. Northern blot analysis detected highest expression of 2.4-, 3.0-, and 7.0-kb transcripts in brain, heart, kidney, and liver, with lower expression in colon and thymus. A 1.1-kb transcript was also detected in PBLs. Yoshinaga et al. (2000) also cloned GL50, which they termed B7RP1. Sequence analysis predicted that the 302-amino acid protein contains a transmembrane domain and 5 conserved cysteine residues. Wang et al. (2000) also cloned this gene, which they termed B7H2. Sequence analysis predicted that the 302-amino acid type I glycoprotein is 21% identical to B7H1 (605402).

ICOS ligand protein family

Belongs to the immunoglobulin superfamily. BTN/MOG family.

ICOS ligand protein name

Recommended name
ICOS ligand
Short name
B7 homolog 2, B7 homologue 2, B7H2, B7-H2, B7-related protein 1, B7RP1, B7RP-1, CD275, GL50, ICOS-L, KIAA0653
Alternative name
B7 homolog 2 B7-like protein Gl50 B7-related protein 1 CD_antigen: CD275

ICOS ligand Gene family protein

ICOS ligand Protein Sequence

Species Human ICOS ligand protein
Length 302
Mass (Da) 33349
Sequence Human ICOS ligand protein sequence
Species Mouse ICOS ligand protein
Length 322
Mass (Da) 35960
Sequence Mouse ICOS ligand protein sequence
Mass (Da)

ICOS ligand Protein Molecular Weight & PI

ICOS ligand precursor (B7 homolog 2) (B7-H2) (B7-like protein Gl50) (B7-related protein 1) (B7RP-1) (CD275 antigen) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 19-302 ICOS ligand.

Molecular weight (Da)


Theoretical pI


ICOS ligand Protein Interaction

Recombinant ICOS ligand Protein Feature

ICOS ligand Protein, Mouse, Recombinant (His Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its binding ability in a functional ELISA. Immobilized mouse B7-H2 at 1 μg/ml (100 μl/well) can bind human ICOS with a linear range of 40-1000 ng/ml.

Recombinant ICOS ligand protein citations

Vγ2Vδ2 T cell Costimulation Increases NK cell Killing of Monocyte‐derived Dendritic Cells
C Cairo, N Surendran, KM Harris…
Rapid de novo generation of antigen specific human B cells with expression of Blimp-1 and AID by in vitro immunization.
Fang, X;Tong, Y;Tian, H;Ning, H;Gao, X;Yao, W;
Exp. Cell Res
Pilot Study of Delayed ICOS/ICOS-L Blockade With αCD40 to Modulate Pathogenic Alloimmunity in a Primate Cardiac Allograft Model
O'Neill, NA;Zhang, T;Braileanu, G;Cheng, X;Hershfeld, A;Sun, W;Reimann, KA;Dahi, S;Kubicki, N;Hassanein, W;Laird, C;Cimeno, A;Azimzadeh, AM;Pierson, RN;
Transplant Direct
Nonimmune cell-derived ICOS ligand functions as a renoprotective αvβ3 integrin-selective antagonist
Koh, KH;Cao, Y;Mangos, S;Tardi, NJ;Dande, RR;Lee, HW;Samelko, B;Altintas, MM;Schmitz, VP;Lee, H;Mukherjee, K;Peev, V;Cimbaluk, DJ;Reiser, J;Hahm, E;
J. Clin. Invest.

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