GAPDH Protein Overview: Sequence, Structure, Function and Protein Interaction

GAPDH Protein Overview

Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) (Dayhoff, 1972).

GAPDH protein family

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

GAPDH protein name

Recommended name
Glyceraldehyde-3-phosphate dehydrogenase
Short name
GAPDH
Alternative name
Peptidyl-cysteine S-nitrosylase GAPDH

GAPDH Protein Sequence

Species Human GAPDH protein
Length 335
Mass (Da) 36053
Sequence Human GAPDH protein sequence
Species Mouse GAPDH protein
Length 333
Mass (Da) 35810
Sequence Mouse GAPDH protein sequence
Species Rat GAPDH protein
Length 333
Mass (Da) 35828
Sequence Rat GAPDH protein sequence

GAPDH Protein Molecular Weight & PI

Glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) (GAPDH) (Peptidyl-cysteine S-nitrosylase GAPDH) (EC 2.6.99.-) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 2-335 Glyceraldehyde-3-phosphate dehydrogenase.

Molecular weight (Da)

35922.02

Theoretical pI

8.58

GAPDH Protein Structure

Crystal structure of human wild-type GAPDH at 1.99 angstroms resolution
Deposited
2014-10-11   Released:  2014-12-03
Deposition Author(s)
Garcin, E.D.
Organism(s)
Homo sapiens
Expression System
Escherichia coli BL21(DE3)
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
1.9900 Å
R-Value Free
0.179
R-Value Work
0.139
4WNC From PDB

Human GAPDH protein Secondary structure

GAPDH Protein Interaction

Recombinant GAPDH Protein Feature

GAPDH Protein, Human, Recombinant (His Tag)

High Purity
> 93 % as determined by SDS-PAGE
Low Endotoxin
Please contact us for more information.

Recombinant GAPDH protein citations

Title
Serum anti‑GAPDH autoantibody levels reflect the severity of cervical lesions: A potential serum biomarker for cervical cancer screening
Year
2019
Author
Xu, M;Kim, H;Kim, S;Ju, W;Kim, Y;Chang, K;Kim, H;
Journal
Oncol Lett
Title
Long-lived mice with reduced growth hormone signaling have a constitutive upregulation of hepatic chaperone-mediated autophagy
Year
2020
Author
Endicott, SJ;Boynton, DN;Beckmann, LJ;Miller, RA;
Journal
Autophagy

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