Fibronectin Protein Overview: Sequence, Structure, Function and Protein Interaction

Fibronectin Protein Overview

Fibronectin-1 belongs to a family of high molecular weight glycoproteins that are present on cell surfaces, in extracellular fluids, connective tissues, and basement membranes. Fibronectins interact with other extracellular matrix proteins and cellular ligands, such as collagen, fibrin, and integrins. Fibronectins are involved in adhesive and migratory processes of cells. Two major forms of fibronectin exist: a plasma soluble form and a cellular form (summary by Muro et al., 2003).

Fibronectin protein name

Recommended name
Fibronectin
Short name
FN||CIG
Aliases
CIG, cold-insoluble globulin, FINC, GFND2, LETS, migration-stimulating factor, MSF
Alternative name
Cold-insoluble globulin Anastellin Ugl-Y1 Ugl-Y2 Ugl-Y3

Fibronectin Gene family protein

Fibronectin Protein Sequence

Species Human Fibronectin protein
Length 2386
Mass (Da) 262625
Sequence Human Fibronectin protein sequence
Species Mouse Fibronectin protein
Length 2477
Mass (Da) 272538
Sequence Mouse Fibronectin protein sequence
Species Rat Fibronectin protein
Length 2477
Mass (Da) 272511
Sequence Rat Fibronectin protein sequence

Fibronectin Protein Molecular Weight & PI

Fibronectin precursor (FN) (Cold-insoluble globulin) (CIG) [Contains: Anastellin; Ugl-Y1; Ugl-Y2; Ugl-Y3] Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 32-2386 Fibronectin.

Molecular weight (Da)

259562.86

Theoretical pI

5.39

Fibronectin Protein Structure

Structure of the Eleventh Type III Domain from Human Fibronectin
Deposited
2015-08-27   Released:  2016-09-14
Deposition Author(s)
Rusnac, D.-V., Mou, T.C., Sprang, S.R., Briknarova, K.
Organism(s)
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.5000 Å
R-Value Free
0.248
R-Value Work
0.193
5DFT From PDB

Human Fibronectin protein Secondary structure

Recombinant Fibronectin Protein Feature

Fibronectin Protein, Human, Recombinant (His Tag)

High Purity
> 97 % as determined by SDS-PAGE.
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to Fibronectin coated plates (2.5 μg/mL and 100 μL/well), approximately 35%-60% cells will adhere specifically after 35 minutes at 37℃.

Recombinant Fibronectin protein citations

Title
Correlation of epididymal protease inhibitor and fibronectin in human semen
Year
2013
Author
Zhang, X;Fang, J;Xu, B;Zhang, S;Su, S;Song, Z;Deng, Y;Wang, H;Zhao, D;Niu, X;Wang, Z;
Journal
PLoS ONE
Title
Defined, serum/feeder-free conditions for expansion and drug screening of primary B-acute lymphoblastic leukemia
Year
2017
Author
Jiang, Z;Wu, D;Ye, W;Weng, J;Lai, P;Shi, P;Guo, X;Huang, G;Deng, Q;Tang, Y;Zhao, H;Cui, S;Lin, S;Wang, S;Li, B;Wu, Q;Li, Y;Liu, P;Pei, D;Du, X;Yao, Y;Li, P;
Journal
Oncotarget

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