Fibronectin Antibody Guideline: Preparation, Dilution and Applications

Fibronectin Antibody Overview

There are 4 Fibronectin antibodies which are validated in multiple tissues with various applications, including ELISA, WB, IP. There are 4 Fibronectin antibody for ELISA, 2 Fibronectin antibody for WB, 2 Fibronectin antibody for IP. Among all these Fibronectin antibodies, there are 1 anti-Fibronectin mouse monoclonal antibodies , 1 anti-Fibronectin rabbit monoclonal antibodies , 2 anti-Fibronectin rabbit polyclonal antibodies . All the Fibronectin anbodies are produced in house and all are in stock. Fibronectin antibody customerized service is available.

Fibronectin Antibody ELISA

Fibronectin antibody ELISA preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Fibronectin Fragment 2 (Catalog#10314-H08H; Ser 607-Pro 1265; NP_997647.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Fibronectin antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

Fibronectin Antibody western blot

Fibronectin Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human Fibronectin Fragment 2 (Catalog#10314-H08H; Ser 607-Pro 1265; CAD91166). Total IgG was purified by Protein A affinity chromatography.

Fibronectin Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

Fibronectin Antibody immunoprecipitation

Fibronectin Antibody immunoprecipitation Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human Fibronectin Fragment 2 (Catalog#10314-H08H; Ser 607-Pro 1265; NP_997647.1).

Fibronectin Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes  

Fibronectin Antibody ICC / IF

Fibronectin Antibody ICC / IF Preparation

Produced in rabbits immunized with purified, recombinant Human Fibronectin (rh Fibronectin; Catalog#10314-H08H; NP_997647.1; Ser607-Pro1265). Fibronectin specific IgG was purified by Human Fibronectin affinity chromatography.

Fibronectin Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:300-1:10000
Notes