DcR3 Protein Overview: Sequence, Structure, Function and Protein Interaction

DcR3 Protein Overview

Pitti et al. (1998) identified ESTs that showed homology to the tumor necrosis factor receptor (TNFR; see 191190) superfamily. Using PCR with primers based on the region of EST consensus, they isolated a cDNA encoding TNFRSF6B, which termed decoy receptor-3 (DCR3), from a human fetal lung cDNA library. The DCR3 protein contains 300 amino acids and has a molecular mass of 35 kD. By Northern blot analysis, Pitti et al. (1998) detected a 1.2-kb transcript in human fetal lung, brain, and liver, and in adult spleen, colon, and lung. Like osteoprotegerin (OPG; 602643), another TNFR superfamily member, DCR3 lacks an apparent transmembrane sequence, indicating that DCR3 may be a secreted, rather than a membrane-associated, molecule. The DCR3 protein shares 31% sequence homology with OPG, and all of the cysteines in the 4 cysteine-rich domains of DCR3 and OPG are conserved. Pitti et al. (1998) stated that DCR3 and OPG define a subset of TNFR family members that function as secreted decoys to modulate ligands that induce apoptosis. Bai et al. (2000) independently identified the TNFRSF6B gene, which they called M68. M68 genomic DNA, mRNA, and protein levels were examined in a series of human gastrointestinal tract tumors. Using M68 immunohistochemistry and a scoring system similar to that used for HER-2/neu (ERBB2; 164870), they found that M68 protein was overexpressed in 30 of 68 (44%) human adenocarcinomas of the esophagus, stomach, colon, and rectum. Tumors examined by Northern blot revealed M68 mRNA highly elevated in a similar fraction of primary tumors from the same gastrointestinal tract regions, as well as in 2 colon adenocarcinoma cell lines. They also found M68 protein to be overexpressed in a substantial number of tumors in which gene amplification could not be detected by fluorescence in situ hybridization or quantitative genomic PCR, suggesting that overexpression of M68 may precede amplification in tumors. Bai et al. (2000) showed that M68 lies within a 4-gene cluster that includes a novel helicase-like gene (NHL; 608833) related to RAD3/ERCC2 (126340), a plasma membrane Ras-related GTPase and a member of the stathmin family (151442), amplification or overexpression of which may also contribute to cell growth and tumor progression.

DcR3 protein name

Recommended name
Tumor necrosis factor receptor superfamily member 6B
Aliases
DcR3, DCR3, M68, TR6
Alternative name
Decoy receptor 3 Decoy receptor for Fas ligand M68

DcR3 Protein Sequence

Species Human DcR3 protein
Length 300
Mass (Da) 32680
Sequence Human DcR3 protein sequence
Species
Length
Mass (Da)
Sequence
Species
Length
Mass (Da)
Sequence

DcR3 Protein Molecular Weight & PI

Tumor necrosis factor receptor superfamily member 6B precursor (Decoy receptor 3) (DcR3) (Decoy receptor for Fas ligand) (M68) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 30-300 Tumor necrosis factor receptor

Molecular weight (Da)

29756.91

Theoretical pI

8.61

DcR3 Protein Structure

Crystal structure of a human light loop mutant in complex with dcr3
Deposited
2013-04-29   Released:  2013-07-10
Deposition Author(s)
Liu, W., Zhan, C., Bonanno, J.B., Sampathkumar, P., Toro, R., Nathenson, S.G., Almo, S.C., New York Structural Genomics Research Consortium (NYSGRC), Atoms-to-Animals: The Immune Function Network (IFN)
Organism(s)
Homo sapiens
Expression System
Escherichia coli, Drosophila
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.2700 Å
R-Value Free
0.238
R-Value Work
0.195
4KGQ From PDB

Human DcR3 protein Secondary structure

DcR3 Protein Interaction

Recombinant DcR3 Protein Feature

DcR3 Protein, Human, Recombinant (hFc Tag)

High Purity
> 85 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.5-3 μg/mL in the presence of 200 ng/mL recombinant human Fas ligand.

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