CLEC4A Protein Overview: Sequence, Structure, Function and Protein Interaction

CLEC4A Protein Overview

Dendritic cells (DCs) are sentinel leukocytes that take up and present carbohydrate-bearing antigens by means of specialized surface receptors. Type I calcium-dependent (C-type) lectin receptors, including macrophage mannose receptor (MMR, or MRC1; 153618) and DEC205 (LY75; 604524), possess multiple calcium-dependent extracellular carbohydrate recognition domains (CRDs) in their N termini. A second family of C-type lectins are type II proteins that have only 1 CRD at the C-terminal end, such as the asialoglycoprotein receptors (ASPGRs; see 108360). By searching an EST database for sequences in DC libraries with a motif shared by the ASPGRs as the probe, Bates et al. (1999) identified a cDNA encoding CLEC4A, which they called DCIR. The deduced 237-amino acid DCIR transmembrane protein, which is 54% identical and 65% homologous to the mouse sequence, lacks a signal sequence but contains a single CRD, a double-cysteine motif at the start of the lectin domain, a potential N-glycosylation site, and a 49-amino acid intracellular immunoreceptor tyrosine-based inhibitory motif (ITIM). Northern blot analysis detected strongest expression of 1.3- and 1.5-kb DCIR transcripts in peripheral blood leukocytes, with moderate expression in spleen, lymph node, and bone marrow, and lower expression in thymus, spinal cord, and trachea; expression was not detected in any other tissues tested. RT-PCR analysis showed that expression of DCIR was restricted to myelomonocytic cells and nonactivated B cells, but not other circulating lymphocytes. Immunohistologic analysis demonstrated that DCIR was expressed in tonsil between the epithelium and around B-cell follicles, but not in germinal centers. Using subtractive cloning of a dendritic cell cDNA library and RACE, Huang et al. (2001) obtained a cDNA encoding CLEC4A, which they termed LLIR. RT-PCR analysis identified 2 splice variants, both of which lack the transmembrane domain but retain the CRD. Western blot analysis showed expression of a 60-kD protein in dendritic cells, B-cell lines, and monocytoid lines.

CLEC4A protein name

Recommended name
C-type lectin domain family 4 member A
Aliases
CD367, DCIR, DDB27
Alternative name
C-type lectin DDB27 C-type lectin superfamily member 6 Dendritic cell immunoreceptor Lectin-like immunoreceptor

CLEC4A Gene family protein

CLEC4A Protein Sequence

Species Human CLEC4A protein
Length 237
Mass (Da) 27512
Sequence Human CLEC4A protein sequence
Species Mouse CLEC4A protein
Length 238
Mass (Da) 27323
Sequence Mouse CLEC4A protein sequence
Species Rat CLEC4A protein
Length 240
Mass (Da) 27404
Sequence Rat CLEC4A protein sequence

CLEC4A Protein Molecular Weight & PI

C-type lectin domain family 4 member A (C-type lectin DDB27) (C-type lectin superfamily member 6) (Dendritic cell immunoreceptor) (Lectin-like immunoreceptor) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 1-237 C-type lectin domain family 4 member A.

Molecular weight (Da)

27512.24

Theoretical pI

6.32

CLEC4A Protein Structure

Crystal structure of human dendritic cell inhibitory receptor (DCIR) C-type lectin domain in ligand-free form
Deposited
2015-12-21   Released:  2016-05-11
Deposition Author(s)
Nagae, M., Yamaguchi, Y.
Organism(s)
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
3.0500 Å
R-Value Free
0.284
R-Value Work
0.253
5B1W From PDB

Human CLEC4A protein Secondary structure

CLEC4A Protein Interaction

Recombinant CLEC4A Protein Feature

CLEC4A Protein, Human, Recombinant (His Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method

Recombinant CLEC4A protein citations

Title
N‐glycan in cockroach allergen regulates human basophil function
Year
2017
Author
Do, DC;Yang, S;Yao, X;Hamilton, RG;Schroeder, JT;Gao, P;
Journal
Immunity, Inflammation and Disease

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