CD55/DAF Protein Overview: Sequence, Structure, Function and Protein Interaction

CD55/DAF Protein Overview

CD55/DAF reagents

The major isoform of DAF, or CD55, is a 70-kD plasma membrane protein that is widely distributed on all blood cells and on endothelial and epithelial tissues. The physiologic role of DAF is to inhibit the complement cascade at the level of the critical C3 (120700) convertase step. By this mechanism, DAF protects autologous cells and tissues from complement-mediated damage and thereby plays a role in preventing or modulating autoimmune disease and inflammation. DAF also serves as a receptor for certain strains of E. coli and certain types of enteroviruses. Variation in DAF forms the basis of the Cromer blood group system (CROM; 613793) (review by Lublin, 2005). In addition to the major membrane-bound isoform of DAF, several other minor soluble and membrane-bound DAF isoforms are produced by alternative splicing (Osuka et al., 2006)

CD55/DAF protein family

Belongs to the receptors of complement activation (RCA) family.

CD55/DAF protein name

Recommended name
Complement decay-accelerating factor
Aliases
CR, CROM, TC
Alternative name
CD antigen CD55

CD55/DAF Gene family protein

CD55/DAF Protein Sequence

Species Human CD55/DAF protein
Length 381
Mass (Da) 41400
Sequence Human CD55/DAF protein sequence
Species Mouse CD55/DAF protein
Length 390
Mass (Da) 42618
Sequence Mouse CD55/DAF protein sequence
Species
Length
Mass (Da)
Sequence

CD55/DAF Protein Molecular Weight & PI

Complement decay-accelerating factor precursor (CD55 antigen) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 35-353 Complement decay-accelerating factor.

Molecular weight (Da)

34964.21

Theoretical pI

7.00

CD55/DAF Protein Structure

Decay accelerating factor (CD55): the structure of an intact human complement regulator.
Deposited
2003-07-16   Released:  2004-01-07
Deposition Author(s)
Lukacik, P., Roversi, P., White, J., Esser, D., Smith, G.P., Billington, J., Williams, P.A., Rudd, P.M., Wormald, M.R., Crispin, M.D.M., Radcliffe, C.M., Dwek, C.M., Evans, D.J., Morgan, B.P., Smith, R.A.G., Lea, S.M.
Organism(s)
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.3000 Å
1OJV From PDB

Human CD55/DAF protein Secondary structure

CD55/DAF Protein Interaction

Recombinant CD55/DAF Protein Feature

CD55/DAF Protein, Human, Recombinant (His Tag)

High Purity
> 95 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its binding ability in a functional ELISA . Immobilized human CD55 at 2 μg/ml (100 μl/well) can bind human CD97 with a linear ranger of 1.28-32 ng/ml.

Recombinant CD55/DAF protein citations

Title
Generation of a felinized Swine endothelial cell line by expression of feline decay-accelerating factor
Year
2015
Author
Izuhara, L;Tatsumi, N;Miyagawa, S;Iwai, S;Watanabe, M;Yamanaka, S;Katsuoka, Y;Nagashima, H;Okano, HJ;Yokoo, T;
Journal
PLoS ONE
Title
Emergence of a Large-Plaque Variant in Mice Infected with Coxsackievirus B3
Year
2016
Author
Wang, Y;Pfeiffer, JK;
Journal
MBio

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