CD46 Antibody Guideline: Preparation, Dilution and Applications

CD46 Antibody Overview

There are 7 CD46 antibodies which are validated in multiple tissues with various applications, including ELISA, IHC-P, FCM, ICC/IF, WB, IP. There are 3 CD46 antibody for ELISA, 3 CD46 antibody for IHC-P, 4 CD46 antibody for FCM, 2 CD46 antibody for ICC/IF, 1 CD46 antibody for WB, 1 CD46 antibody for IP. Among all these CD46 antibodies, there are 5 anti-CD46 mouse monoclonal antibodies , 1 anti-CD46 rabbit monoclonal antibodies , 1 anti-CD46 rabbit polyclonal antibodies . All the CD46 anbodies are produced in house and all are in stock. CD46 antibody customerized service is available.

CD46 Antibody ELISA

CD46 antibody ELISA preparation

Produced in rabbits immunized with purified, recombinant Human CD46 (rh CD46; Catalog#12239-H08H; EAW93471.1; Met1-Asp328). Total IgG was purified by Protein A affinity chromatography.

CD46 antibody ELISA validated

Application ELISA
Dilution 1:1000-1:2000
Notes  

CD46 Antibody western blot

CD46 Antibody western blot

CD46 Antibody western blot Preparation

Produced in rabbits immunized with purified, recombinant Human CD46 (rh CD46; Catalog#12239-H08H; EAW93471.1; Met 1-Asp 328). CD46 specific IgG was purified by Human CD46 affinity chromatography.

CD46 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

CD46 Antibody flow cytometry

CD46 Antibody flow cytometry

CD46 Antibody immunohistochemistry

CD46 Antibody immunohistochemistry

CD46 Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD46 (rh CD46; Catalog#12239-H08H; EAW93471.1; Met 1-Asp 328). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD46 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

CD46 Antibody immunoprecipitation

CD46 Antibody immunoprecipitation

CD46 Antibody immunoprecipitation Preparation

Produced in rabbits immunized with purified, recombinant Human CD46 (rh CD46; Catalog#12239-H08H; EAW93471.1; Met 1-Asp 328). CD46 specific IgG was purified by Human CD46 affinity chromatography.

CD46 Antibody immunoprecipitation Validated

Application IP
Dilution 1-2 μL/mg of lysate
Notes  

CD46 Antibody ICC / IF

CD46 Antibody ICC / IF

CD46 Antibody ICC / IF Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD46 (rh CD46; Catalog#12239-H08H; EAW93471.1; Met1-Asp328). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD46 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

CD46 antibody citations

Title
Complement regulatory proteins (CD46, 55 and 59) expressed on Schwann cells: Immune targets in demyelinating neuropathies?
Year
2014
Author
Miyaji, K;Paul, F;Shahrizaila, N;Umapathi, T;Yuki, N;
Journal
J. Neuroimmunol.
Title
Complement regulatory proteins (CD46, 55 and 59) expressed on Schwann cells: Immune targets in demyelinating neuropathies?
Year
2014
Author
Miyaji, K;Paul, F;Shahrizaila, N;Umapathi, T;Yuki, N;
Journal
J. Neuroimmunol.