CD45 Protein Overview: Sequence, Structure, Function and Protein Interaction

CD45 Protein Overview

The tyrosine phosphatase CD45 (151460) plays a key role in lymphocyte activation. Its expression is required for proximal signaling events following triggering of the T- and B-cell antigen receptors in human and mouse, and in T cells, the enzymatic phosphatase activity of CD45 is required to link the T cell receptor/CD3 complex to the intracellular signaling machinery. Schraven et al. (1994) described the cDNA and protein sequence of a 32-kD transmembrane phosphoprotein, which they called LPAP (lymphocyte phosphatase associated phosphoprotein), that noncovalently interacts with CD45 in human lymphocytes. The interaction of LPAP and CD45 is mediated by the transmembrane regions of both molecules and seems to be required for stable protein expression of LPAP. The mouse homolog is a 30-kD transmembrane protein.

CD45 protein family

Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.

CD45 protein name

Recommended name
Receptor-type tyrosine-protein phosphatase C
Aliases
GP180, LCA, T200
Alternative name
Leukocyte common antigen T200 CD_antigen: CD45

CD45 Gene family protein

Protein tyrosine phosphatases, receptor type

CD45 Protein Sequence

Species Human CD45 protein
Length 1306
Mass (Da) 147486
Sequence Human CD45 protein sequence
Species Mouse CD45 protein
Length 1293
Mass (Da) 144837
Sequence Mouse CD45 protein sequence
Species Rat CD45 protein
Length 1273
Mass (Da) 143269
Sequence Rat CD45 protein sequence

CD45 Protein Molecular Weight & PI

Receptor-type tyrosine-protein phosphatase C precursor (EC 3.1.3.48) (Leukocyte common antigen) (L-CA) (T200) (CD45 antigen) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 26-1306 Receptor-type tyrosine-protein

Molecular weight (Da)

144589.99

Theoretical pI

5.80

CD45 Protein Structure

Crystal structure of human CD45 extracellular region, domains d1-d2
Deposited
2015-11-10   Released:  2016-03-23
Deposition Author(s)
Chang, V.T., Fernandes, R.A., Ganzinger, K.A., Lee, S.F., Siebold, C., McColl, J., Jonsson, P., Palayret, M., Harlos, K., Coles, C.H., Jones, E.Y., Lui, Y., Huang, E., Gilbert, R.J.C., Klenerman, D., Aricescu, A.R., Davis, S.J.
Organism(s)
Homo sapiens
Expression System
Homo sapiens
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.3000 Å
R-Value Free
0.238
R-Value Work
0.202
5FN7 From PDB

Human CD45 protein Secondary structure

CD45 Protein Interaction

Recombinant CD45 Protein Feature

CD45 Protein, Human, Recombinant (hFc Tag)

High Purity
> 90 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by its ability to bind biotinylated human Galectin-1 (Cat:10290-HNAE-E) in a functional ELISA.

Recombinant CD45 protein citations

Title
Kindlin-3 Is Essential for the Resting α4β1 Integrin-mediated Firm Cell Adhesion under Shear Flow Conditions
Year
2016
Author
Lu, L;Lin, C;Yan, Z;Wang, S;Zhang, Y;Wang, S;Wang, J;Liu, C;Chen, J;
Journal
J. Biol. Chem.

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