CD4 Protein Overview: Sequence, Structure, Function and Protein Interaction

CD4 Protein Overview

CD4 reagents

CD4 is a cell surface glycoprotein that is expressed on subsets of thymocytes and mature T lymphocytes, as well as on monocytes and macrophages. CD4 plays an important role in T-helper (Th) cell development and activation (summary by Zeitlmann et al., 2001).

CD4 protein name

Recommended name
T-cell surface glycoprotein CD4
Alternative name
T-cell surface antigen T4/Leu-3 CD antigen CD4

CD4 Gene family protein

CD4 Protein Sequence

Species Human CD4 protein
Length 458
Mass (Da) 51111
Sequence Human CD4 protein sequence
Species Mouse CD4 protein
Length 457
Mass (Da) 51297
Sequence Mouse CD4 protein sequence
Species Rat CD4 protein
Length 457
Mass (Da) 51438
Sequence Rat CD4 protein sequence

CD4 Protein Molecular Weight & PI

T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (CD4 antigen) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 26-458 T-cell surface glycoprotein CD4.

Molecular weight (Da)

48399.23

Theoretical pI

9.52

CD4 Protein Structure

CD4 endocytosis motif bound to the Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase
Deposited
2007-10-30   Released:  2008-01-01
Deposition Author(s)
Garron, M.-L., Arold, S.T.
Organism(s)
Homo sapiens
Expression System
Escherichia coli BL21
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.8200 Å
R-Value Free
0.307
R-Value Work
0.241
3B71 From PDB

Human CD4 protein Secondary structure

CD4 Protein Interaction

Recombinant CD4 Protein Feature

CD4 Protein, Human, Recombinant (His Tag)

High Purity
> 90 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to CD4-coated plates (0.8 μg/mL, 100 μL/well), approximately >40% will adhere specifically.

Recombinant CD4 protein citations

Title
Anti-serpin antibody-mediated regulation of proteases in autoimmune diabetes
Year
2013
Author
Baldzizhar, R;Fedorchuk, C;Jha, M;Rathinam, C;Henegariu, O;Czyzyk, J;
Journal
J. Biol. Chem.
Title
Design, Synthese und Analytik peptidischer CD4-Mimetika sowie Analyse ihrer Bindung an das HIV-Hüllprotein gp120
Year
2013
Author
Meier, J;
Journal
Thesis
Title
Design, Synthese und Analytik peptidischer CD4-Mimetika sowie Analyse ihrer Bindung an das HIV-Hüllprotein gp120
Year
2013
Author
Meier, J;
Journal
Thesis
Title
Engineering of HIV gp120 by yeast surface display for neutralizing antibody characterization and immunogen design
Year
2013
Author
Mata-Fink, J;
Journal
Thesis
Title
Functional ligands to target molecules
Year
2013
Author
GW Jackson
Journal
US Patent App. 13/748,566
Title
Mimicking protein–protein interactions through peptide–peptide interactions: HIV-1 gp120 and CXCR4
Year
2013
Author
A Groß, K Möbius, C Haußner, N Donhauser…
Journal
Frontiers in immunology
Title
Rapid conformational epitope mapping of anti-gp120 antibodies with a designed mutant panel displayed on yeast
Year
2013
Author
Mata-Fink, J;Kriegsman, B;Yu, HX;Zhu, H;Hanson, MC;Irvine, DJ;Wittrup, KD;
Journal
J. Mol. Biol.
Title
Antigenic and 3D structural characterization of soluble X4 and hybrid X4-R5 HIV-1 Env trimers
Year
2014
Author
Arnold, P;Himmels, P;Weiß, S;Decker, TM;Markl, J;Gatterdam, V;Tampé, R;Bartholomäus, P;Dietrich, U;Dürr, R;
Journal
Retrovirology
Title
Blocking interaction of viral gp120 and CD4-expressing T cells by single-stranded DNA aptamers
Year
2014
Author
Zhao, N;Pei, SN;Parekh, P;Salazar, E;Zu, Y;
Journal
Int. J. Biochem. Cell Biol.
Title
Deletion of the highly conserved N-glycan at Asn260 of HIV-1 gp120 affects folding and lysosomal degradation of gp120, and results in loss of viral infectivity
Year
2014
Author
Mathys, L;François, KO;Quandte, M;Braakman, I;Balzarini, J;
Journal
PLoS ONE
Title
The role of N -glycans of HIV-1 gp41 in virus infectivity and susceptibility to the suppressive effects of carbohydrate-binding agents
Year
2014
Author
Mathys, L;Balzarini, J;
Journal
Retrovirology
Title
Off-target-free gene delivery by affinity-purified receptor-targeted viral vectors
Year
2015
Author
Münch, RC;Muth, A;Muik, A;Friedel, T;Schmatz, J;Dreier, B;Trkola, A;Plückthun, A;Büning, H;Buchholz, CJ;
Journal
Nat Commun
Title
An HIV gp120-CD4 Immunogen Does Not Elicit Autoimmune Antibody Responses in Cynomolgus Macaques
Year
2016
Author
Schwartz, JA;Prado, I;Misamore, J;Weiss, D;Francis, J;Pal, R;Huaman, M;Cristillo, A;Lewis, GK;Gallo, RC;DeVico, AL;Fouts, TR;
Journal
Clin. Vaccine Immunol.
Title
Highly sensitive amperometric detection of cardiac troponin I using sandwich aptamers and screen-printed carbon electrodes
Year
2017
Author
Jo, H;Her, J;Lee, H;Shim, YB;Ban, C;
Journal
Talanta
Title
Comparison of the genotypic and phenotypic properties of HIV-1 standard subtype B and subtype B/B' env molecular clones derived from infections in China
Year
2018
Author
Xie, H;Nie, J;Chen, Q;Huang, W;Wang, Y;
Journal
Emerg Microbes Infect
Title
Optimization of Enzymatic Antibody Fragmentation for Yield, Efficiency, and Binding Affinity
Year
2019
Author
Kinman, AWL;Pompano, RR;
Journal
Bioconjug. Chem.
Title
CD40 Enhances Type I Interferon Responses Downstream of CD47 Blockade, Bridging Innate and Adaptive Immunity
Year
2019
Author
de Silva, S;Fromm, G;Shuptrine, CW;Johannes, K;Patel, A;Yoo, KJ;Huang, K;Schreiber, TH;
Journal
Cancer Immunol Res

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