CD39 Protein Overview: Sequence, Structure, Function and Protein Interaction

CD39 Protein Overview

Endothelial cells have the ability to regulate platelet activation, in part by the surface expression of ATP diphosphohydrolase (ATPDase; EC 3.6.1.5). ATPDase hydrolyzes extracellular ATP and ADP to AMP, which is further converted to adenosine by another enzyme, 5-prime nucleotidase. ADP is a powerful agonist for platelet recruitment and adhesion; adenosine is an antagonist of these processes. Kaczmarek et al. (1996) demonstrated that CD39, a B-cell activation marker previously characterized by Maliszewski et al. (1994), encodes vascular ATPDase. They isolated the cDNA of human ATPDase/CD39 by RT-PCR using RNA from human umbilical endothelial cells. They expressed this cDNA in COS-7 cells and confirmed that it is expressed on the cell surface, hydrolyzes both ATP and ADP, and inhibits platelet aggregation. CD39 was found to have both immunologic identity to, and functional characteristics of, vascular ATPDase. By Northern blot analysis, Chadwick and Frischauf (1998) found that CD39 is expressed as a major 3.2- and a minor 3.6-kb mRNA in several tissues. Additional bands were observed in a few tissues.

CD39 protein family

Belongs to the GDA1/CD39 NTPase family.

CD39 protein name

Recommended name
Ectonucleoside triphosphate diphosphohydrolase 1
Short name
NTPDase 1
Aliases
ATPDase, NTPDase-1, SPG64
Alternative name
Ecto-ATP diphosphohydrolase 1 Ecto-ATPDase 1 Ecto-ATPase 1 Ecto-apyrase Lymphoid cell activation antigen CD antigen CD39

CD39 Protein Sequence

Species Human CD39 protein
Length 510
Mass (Da) 57965
Sequence Human CD39 protein sequence
Species Mouse CD39 protein
Length 510
Mass (Da) 57205
Sequence Mouse CD39 protein sequence
Species Rat CD39 protein
Length 511
Mass (Da) 57408
Sequence Rat CD39 protein sequence

CD39 Protein Molecular Weight & PI

Ectonucleoside triphosphate diphosphohydrolase 1 (EC 3.6.1.5) (NTPDase 1) (Ecto-ATP diphosphohydrolase 1) (Ecto-ATPDase 1) (Ecto-ATPase 1) (Ecto-apyrase) (Lymphoid cell activation antigen) (CD39 antigen) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 1-510 Ectonucleoside triphosphate

Molecular weight (Da)

57964.51

Theoretical pI

5.93

CD39 Protein Interaction

Recombinant CD39 Protein Feature

CD39 Protein, Human, Recombinant (ECD, His Tag)

High Purity
> 90 % as determined by SDS-PAGE.
Low Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
High Activity
Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The specific activity is >5000 pmol/min/µg.

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