CD38 Protein Overview: Sequence, Structure, Function and Protein Interaction

CD38 Protein Overview

By immunoscreening a lymphocyte cDNA library transiently expressed in COS cells, Jackson and Bell (1990) obtained a cDNA encoding CD38. Immunoprecipitation and SDS-PAGE analysis showed expression of a 46-kD protein under reducing conditions and a 42-kD protein under nonreducing conditions, both of which are larger than the predicted 34-kD protein, suggesting that CD38 is glycosylated. Northern blot analysis detected various transcripts in B and T lymphoblast cell lines, but no expression was found in resting lymphocytes. The predicted 300-amino acid type II transmembrane protein has a short N-terminal cytoplasmic tail and 4 C-terminal extracellular N-glycosylation sites. It lacks homology to immunoglobulin (Ig) superfamily or major histocompatibility complex (MHC) molecules.

CD38 protein family

Belongs to the ADP-ribosyl cyclase family.

CD38 protein name

Recommended name
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
Aliases
ADP-ribosyl cyclase 1, NAD(+) nucleosidase
Alternative name
2'-phospho-ADP-ribosyl cyclase 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase EC 2.4.99.20 2'-phospho-cyclic-ADP-ribose transferase ADP-ribosyl cyclase 1 ADPRC 1 Cyclic ADP-ribose hydrolase 1 cADPr hydrolase 1 T10 CD antigen CD38

CD38 Protein Sequence

Species Human CD38 protein
Length 300
Mass (Da) 34328
Sequence Human CD38 protein sequence
Species Mouse CD38 protein
Length 304
Mass (Da) 34408
Sequence Mouse CD38 protein sequence
Species Rat CD38 protein
Length 303
Mass (Da) 34436
Sequence Rat CD38 protein sequence

CD38 Protein Molecular Weight & PI

ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (EC 3.2.2.6) (2'-phospho-ADP-ribosyl cyclase) (2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase) (EC 2.4.99.20) (2'-phospho-cyclic-ADP-ribose transferase) (ADP-ribosyl cyclase 1) (ADPRC 1) (Cyclic ADP-ribose hydrolase 1) (cADPr hydrolase 1) (T10) (CD38 antigen) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 1-300 ADP-ribosyl cyclase/cyclic ADP-ribose

Molecular weight (Da)

34328.43

Theoretical pI

7.85

CD38 Protein Structure

Crystal structure of human CD38 extracellular domain complexed with a covalent intermediate, ara-F-ribose-5'-phosphate
Deposited
2008-07-29   Released:  2008-11-04
Deposition Author(s)
Liu, Q., Kriksunov, I.A., Jiang, H., Graeff, R., Lin, H., Lee, H.C., Hao, Q.
Organism(s)
Homo sapiens
Expression System
Pichia pastoris
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
2.0100 Å
R-Value Free
0.272
R-Value Work
0.201
3DZF From PDB

Human CD38 protein Secondary structure

CD38 Protein Interaction

Recombinant CD38 Protein Feature

CD38 Protein, Human, Recombinant (ECD, His Tag)

High Purity
> 97 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
1. Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDPribose.The specific activity is > 2,500 pmoles/min/μg. 2. Measured by its binding ability in a functional ELISA. Immobilized Human CD38 His (Cat:10818-H08H) at 2 μg/ml (100 μl/well) can bind Mouse Anti-CD38 Antibody (Cat:10818-MM03), the EC50 of Mouse Anti-CD38 Antibody is 30-120 ng/mL.

Recombinant CD38 protein citations

Title
Novel anti-CD38 humanized mAb SG003 possessed enhanced cytotoxicity in lymphoma than Daratumumab via antibody-dependent cell-mediated cytotoxicity
Year
2019
Author
Yu, T;Qiao, C;Lv, M;Tang, L;
Journal
BMC Biotechnol.
Title
Blocking CD38-driven fratricide among T cells enables effective antitumor activity by CD38-specific chimeric antigen receptor T cells
Year
2019
Author
Gao, Z;Tong, C;Wang, Y;Chen, D;Wu, Z;Han, W;
Journal
J Genet Genomics
Title
Blocking CD38-driven fratricide among T cells enables effective antitumor activity by CD38-specific chimeric antigen receptor T cells
Year
2019
Author
Gao, Z;Tong, C;Wang, Y;Chen, D;Wu, Z;Han, W;
Journal
J Genet Genomics
Title
A novel approach to remove interference of therapeutic monoclonal antibody with serum protein electrophoresis
Year
2019
Author
Liu, L;Shurin, MR;Wheeler, SE;
Journal
Clin. Biochem.
Title
Complete Depletion of Daratumumab Interference in Serum Samples from Plasma Cell Myeloma Patients Improves the Detection of Endogenous M-Proteins in a Preliminary Study
Year
2020
Author
Vakili, H;Koorse Germans, S;Dong, X;Kansagra, A;Patel, H;Muthukumar, A;Hashim, I;
Journal
Diagnostics

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