CD3 epsilon/CD3e Protein Overview: Sequence, Structure, Function and Protein Interaction

CD3 epsilon/CD3e Protein Overview

CD3 epsilon/CD3e reagents

The T-cell antigen receptor is comprised of multiple subunits: alpha (see 186880), beta (see 186930), gamma (186740), delta (186790), epsilon, and zeta (186780). The last 4 constitute T3. The first 2 are linked by disulfide bonds. The gamma and delta T3 membrane proteins are glycoproteins; T3-epsilon is not glycosylated. Gold et al. (1986) cloned cDNA corresponding to the T3-epsilon gene, T3E.

CD3 epsilon/CD3e protein name

Recommended name
T-cell surface glycoprotein CD3 epsilon chain
Alternative name
T-cell surface antigen T3/Leu-4 epsilon chain CD antigen CD3e

CD3 epsilon/CD3e Protein Sequence

Species Human CD3 epsilon/CD3e protein
Length 207
Mass (Da) 23147
Sequence Human CD3 epsilon/CD3e protein sequence
Species Mouse CD3 epsilon/CD3e protein
Length 189
Mass (Da) 21393
Sequence Mouse CD3 epsilon/CD3e protein sequence
Species
Length
Mass (Da)
Sequence

CD3 epsilon/CD3e Protein Molecular Weight & PI

T-cell surface glycoprotein CD3 epsilon chain precursor (T-cell surface antigen T3/Leu-4 epsilon chain) (CD3e antigen) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 23-207 T-cell surface glycoprotein CD3 epsilon

Molecular weight (Da)

20737.37

Theoretical pI

5.78

CD3 epsilon/CD3e Protein Structure

CRYSTAL STRUCTURE OF THE TANDEM SH2 DOMAIN OF THE SYK KINASE BOUND TO A DUALLY TYROSINE-PHOSPHORYLATED ITAM
Deposited
1998-03-31   Released:  1998-10-21
Deposition Author(s)
Fuetterer, K., Waksman, G.
Organism(s)
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
3.0000 Å
R-Value Free
0.317
R-Value Work
0.226
1A81 From PDB

Human CD3 epsilon/CD3e protein Secondary structure

CD3 epsilon/CD3e Protein Interaction

Recombinant CD3 epsilon/CD3e Protein Feature

CD3 epsilon/CD3e Protein, Human, Recombinant (His Tag)

High Purity
> 80 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Using the Octet RED System, the affinity constant (Kd) of CD3 epsilon / CD3e Protein, Human, Recombinant (His Tag) (Cat: 10977-H08H) bound to Human CD3 (humanized OKT3) Antibody-Human IgG4 was 3.8 nM.

Recombinant CD3 epsilon/CD3e protein citations

Title
PD-1+ Tim3+ tumor-infiltrating CD8 T cells sustain the potential for IFN-γ production, but lose cytotoxic activity in ovarian cancer
Year
2020
Author
Sawada, M;Goto, K;Morimoto-Okazawa, A;Haruna, M;Yamamoto, K;Yamamoto, Y;Nakagawa, S;Hiramatsu, K;Matsuzaki, S;Kobayashi, E;Kawashima, A;Hirata, M;Iwahori, K;Kimura, T;Ueda, Y;Kimura, T;Wada, H;
Journal
Int. Immunol.

Bulk Order of Recombinant CD3 epsilon/CD3e Protein

Please Leave Us a Message if you have any questions regarding bulk price quote of our products on the website, our customer specialist will get back to you in 24 hours by email.

Custom Recombinant CD3 epsilon/CD3e Protein Service

Custom Recombinant Protein Production Service Features

  • Over 10 years' experience for 6000+ recombinant proteins production.
  • One-stop service from gene synthesis and vector construction to protein expression and purification.
  • Multiple protein expression systems: bacterial, yeast, baculovirus-insect and mammalian expression system.
  • Multiple purification systems (30+) to choose.
  • High R & D ability with over 1000 new proteins per year and quick problem-solving ability.
  • Close to 100 bioreactors with various volume between 2-1000 L to guarantee high-throughput and large-scale production.

Custom Recombinant Protein Production Service Advantages

  • High-efficiency expression vectors
  • High cell density culturing
  • Proprietary medium formulation
  • >6000 proteins expression and purification experience
  • High-efficiency expression vectors
  • High cell density culturing

Recombinant Protein Production Service Description

We offer one-stop services for recombinant protein production using our advanced protein expression platform technologies. Our full services include protein gene synthesis, protein codon optimization, protein expression vector design, large scale cell culture and fermentation, protein purification, and protein quality control testing. We offer significant cost saving advantages and record speed in protein expression and bulk production. We also have extensive experiences and expertise in handling various types of recombinant protein purification projects, with or without purification tags.