CD19 Antibody Guideline: Preparation, Dilution and Applications

CD19 Antibody Overview

There are 12 CD19 antibodies which are validated in multiple tissues with various applications, including ELISA(Cap), WB, ELISA, ELISA(Det), FCM, ICC/IF, IHC-P, IP. There are 1 CD19 antibody for ELISA(Cap), 1 CD19 antibody for WB, 4 CD19 antibody for ELISA, 1 CD19 antibody for ELISA(Det), 3 CD19 antibody for FCM, 1 CD19 antibody for ICC/IF, 3 CD19 antibody for IHC-P, 2 CD19 antibody for IP. Among all these CD19 antibodies, there are 7 anti-CD19 mouse monoclonal antibodies , 4 anti-CD19 rabbit monoclonal antibodies , 1 anti-CD19 rabbit polyclonal antibodies . All the CD19 anbodies are produced in house and all are in stock. CD19 antibody customerized service is available.

CD19 Antibody ELISA

CD19 Antibody flow cytometry

CD19 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD19 (rh CD19; Catalog#11880-H08H; P15391; Met1-Lys291). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD19 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

CD19 Antibody immunohistochemistry

CD19 Antibody immunohistochemistry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD19 (rh CD19; Catalog#11880-H08H; P15391; Met1-Lys291). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD19 Antibody immunohistochemistry Validated

Application IHC
Dilution 1:50-1:200
Notes  

CD19 Antibody immunoprecipitation

CD19 Antibody immunoprecipitation Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD19 (rh CD19; Catalog#11880-H08H; P15391; Met1-Lys291).

CD19 Antibody immunoprecipitation Validated

Application IP
Dilution 1-4 μL/mg of lysate
Notes