BMP-2 Antibody Guideline: Preparation, Dilution and Applications

BMP-2 Antibody Overview

There are 6 BMP-2 antibodies which are validated in multiple tissues with various applications, including ELISA(Det), WB, ELISA, IP, FCM, ICC/IF, ELISA(Cap). There are 1 BMP-2 antibody for ELISA(Det), 1 BMP-2 antibody for WB, 2 BMP-2 antibody for ELISA, 1 BMP-2 antibody for IP, 1 BMP-2 antibody for FCM, 2 BMP-2 antibody for ICC/IF, 1 BMP-2 antibody for ELISA(Cap). Among all these BMP-2 antibodies, there are 4 anti-BMP-2 mouse monoclonal antibodies , 1 anti-BMP-2 rabbit monoclonal antibodies , 1 anti-BMP-2 rabbit polyclonal antibodies . All the BMP-2 anbodies are produced in house and all are in stock. BMP-2 antibody customerized service is available.

BMP-2 Antibody ELISA

BMP-2 Antibody western blot

BMP-2 Antibody western blot Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BMP-2 (rh BMP-2 ; Catalog#10426-HNAE; NP_001191.1; Gln 283-Arg 396). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

BMP-2 Antibody western blot Validated

Application WB
Dilution 1:500-1:1000
Notes  

BMP-2 Antibody flow cytometry

BMP-2 Antibody flow cytometry Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BMP-2 (rh BMP-2; Catalog#10426-HNAE; NP_001191.1; Gln283-Arg396). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

BMP-2 Antibody flow cytometry Validated

Application FC
Dilution 1:25-1:100
Notes  

BMP-2 Antibody immunoprecipitation

BMP-2 Antibody immunoprecipitation Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BMP-2 (rh BMP-2 ; Catalog#10426-HNAE; NP_001191.1; Gln 283-Arg 396). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

BMP-2 Antibody immunoprecipitation Validated

Application IP
Dilution 0.2-1 μL/mg of lysate
Notes  

BMP-2 Antibody ICC / IF

BMP-2 Antibody ICC / IF Preparation

This antibody was obtained from a rabbit immunized with purified, recombinant Human BMP-2 (rh BMP-2; Catalog#10426-HNAE; NP_001191.1; Gln283-Arg396).

BMP-2 Antibody ICC / IF Validated

Application ICC_IF
Dilution 1:20-1:100
Notes  

BMP-2 antibody citations

Title
Heterologous Secretory Expression and Characterization of Dimerized Bone Morphogenetic Protein 2 in Bacillus subtilis
Year
2017
Author
Hanif, MU;Gul, R;Hanif, MI;Hashmi, AA;
Journal
BioMed Research International
Title
Small Ubiquitin-Like Modifier Protein 3 Enhances the Solubilization of Human Bone Morphogenetic Protein 2 in E. coli
Year
2018
Author
Hanif, MU;Yaseen, A;Gul, R;Mirza, MU;Nawaz, MH;Ahmed, SS;Aziz, S;Chaudhary, S;Khan, AA;Shoaib, M;
Journal
Appl. Biochem. Biotechnol.