Argonaute-2 Protein Overview: Sequence, Structure, Function and Protein Interaction

Argonaute-2 Protein Overview

Argonaute-2 reagents

Binding of microRNA (miRNA) to mRNA within the RNA-induced silencing complex (RISC) leads to either translational inhibition or destruction of the target mRNA. EIF2C2, or Argonaute-2, is a core RISC component that has both mRNA inhibition and degradation functions (summary by O''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''''Carroll et al., 2007).

Argonaute-2 protein family

Belongs to the argonaute family. Ago subfamily.

Argonaute-2 protein name

Recommended name
Protein argonaute-2
Short name
Argonaute2||hAgo2
Aliases
argonaute 2, hAGO2, LINC00980, Q10
Alternative name
Argonaute RISC catalytic component 2 Eukaryotic translation initiation factor 2C 2 eIF-2C 2 eIF2C 2 PAZ Piwi domain protein PPD Protein slicer

Argonaute-2 Gene family protein

Argonaute/PIWI family
Approved symbol Common name
AGO1 AGO1 protein, recombinant
AGO3 AGO3 protein, recombinant

Argonaute-2 Protein Sequence

Species Human Argonaute-2 protein
Length 859
Mass (Da) 97208
Sequence Human Argonaute-2 protein sequence
Species Mouse Argonaute-2 protein
Length 860
Mass (Da) 97304
Sequence Mouse Argonaute-2 protein sequence
Species Rat Argonaute-2 protein
Length 860
Mass (Da) 97318
Sequence Rat Argonaute-2 protein sequence

Argonaute-2 Protein Molecular Weight & PI

Protein argonaute-2 (EC 3.1.26.n2) (Argonaute2) (hAgo2) (Argonaute RISC catalytic component 2) (Eukaryotic translation initiation factor 2C 2) (eIF-2C 2) (eIF2C 2) (PAZ Piwi domain protein) (PPD) (Protein slicer) Homo sapiens (Human).

The parameters have been computed for the following feature

FT CHAIN 1-859 Protein argonaute-2.

Molecular weight (Da)

97208.11

Theoretical pI

9.32

Argonaute-2 Protein Structure

Crystal structure of MID domain from hAGO2
Deposited
2010-02-17   Released:  2010-05-26
Deposition Author(s)
Frank, F., Sonenberg, N., Nagar, B.
Organism(s)
Homo sapiens
Expression System
Escherichia coli
Experimental Data Snapshot
Method
X-RAY DIFFRACTION
Resolution
1.6910 Å
R-Value Free
0.209
R-Value Work
0.174
3LUC From PDB

Human Argonaute-2 protein Secondary structure

Argonaute-2 Protein Interaction

Recombinant Argonaute-2 Protein Feature

Argonaute-2 Protein, Human, Recombinant (His Tag)

High Purity
> 85 % as determined by SDS-PAGE
Low Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
High Activity
Human AGO2 (11079-H07B) can bind Let-7a RNA and cleave target RNA (21nt).

Recombinant Argonaute-2 protein citations

Title
Quantitative functions of Argonaute proteins in mammalian development
Year
2012
Author
Wang, D;Zhang, Z;O'Loughlin, E;Lee, T;Houel, S;O'Carroll, D;Tarakhovsky, A;Ahn, NG;Yi, R;
Journal
Genes Dev.
Title
The ubiquitin ligase mLin41 temporally promotes neural progenitor cell maintenance through FGF signaling
Year
2012
Author
Chen, J;Lai, F;Niswander, L;
Journal
Genes Dev.
Title
Tools for investigation of the RNA endonuclease activity of mammalian Argonaute2 protein
Year
2012
Author
Yang, N;Cao, Y;Han, P;Zhu, X;Sun, L;Li, G;
Journal
Anal. Chem.
Title
Wig1 prevents cellular senescence by regulating p21 mRNA decay through control of RISC recruitment
Year
2012
Author
Kim, BC;Lee, HC;Lee, JJ;Choi, CM;Kim, DK;Lee, JC;Ko, YG;Lee, JS;
Journal
EMBO J.
Title
A novel graphene oxide-based fluorescence assay for RNA endonuclease activity of mammalian Argonaute2 protein
Year
2013
Author
Li, F;Chen, M;Sun, X;Wang, X;Li, P;
Journal
Sensors and Actuators B: Chemical
Title
A novel strategy to inhibit the reproduction and translation of hepatitis C virus
Year
2013
Author
Duan, A;Ning, L;Li, C;Hou, Y;Yang, N;Sun, L;Li, G;
Journal
Sci China Life Sci
Title
MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation
Year
2014
Author
Zhang, X;Zuo, X;Yang, B;Li, Z;Xue, Y;Zhou, Y;Huang, J;Zhao, X;Zhou, J;Yan, Y;Zhang, H;Guo, P;Sun, H;Guo, L;Zhang, Y;Fu, XD;
Journal
Cell
Title
DICER-ARGONAUTE2 complex in continuous fluorogenic assays of RNA interference enzymes
Year
2015
Author
Bernard, MA;Wang, L;Tachado, SD;
Journal
PLoS ONE
Title
Preassembled Single-Stranded RNA-Argonaute Complexes: A Novel Method to Silence Genes in Cryptosporidium
Year
2015
Author
Castellanos-Gonzalez, A;Perry, N;Nava, S;White, AC;
Journal
J. Infect. Dis.
Title
Bio-functional surfaces for the immunocapture of AGO2-bound microRNAs
Year
2016
Author
Vaghi, V;Potrich, C;Lunelli, L;Facci, P;Pasquardini, L;Vanzetti, L;Pederzolli, C;
Journal
Colloids Surf B Biointerfaces
Title
Post-transcriptional gene silencing mediated by microRNAs is controlled by nucleoplasmic Sfpq
Year
2017
Author
Bottini, S;Hamouda-Tekaya, N;Mategot, R;Zaragosi, LE;Audebert, S;Pisano, S;Grandjean, V;Mauduit, C;Benahmed, M;Barbry, P;Repetto, E;Trabucchi, M;
Journal
Nat Commun
Title
Cryptosporidium parvum Subtilisin-Like Serine Protease (SUB1) Is Crucial for Parasite Egress from Host Cells
Year
2019
Author
Nava, S;White, AC;Castellanos-González, A;
Journal
Infect. Immun.
Title
Detection of MicroRNAs Released from Argonautes
Year
2020
Author
Min, KW;Evans, JG;Won, EC;Yoon, JH;
Journal
Methods Mol. Biol.

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