SARS-CoV-2 Spike Mutant Protein

SARS-CoV-2 Mutation in Danish Mink
Recombinant Spike RBD Mutant (Y453F)

An unprecedented mink cullhas been ordered in Denmark amid the outbreak of SARS-COV-2 in these farmed animals. The plan was announced after scientists discovered a widespread Y453F mutation in the spike protein, that has been passed from animal to humans.

This mutation is of particular concern, because it occurs at a conservative domain of the receptor binding domain (RBD) directly involved in ACE2 binding. Results from some preliminary studies suggest the Y453F mutation affects the ability of the Spike protein to bind with ACE2, while others demonstratethat the mutated spike can escape from detection from a commercial anti-S antibody.

Although there is still no clear evidence indicating this mutation, or any other mutation like the popular D614G, have any clinical significance, the characteristics of the mutations need to be thoroughly investigated in the context of vaccine and antibody therapy.

Sino Biological has launched the recombinant Y453F RBD protein. This product is the newest addition to a large library of recombinant spike variants (full list here). These proteins can be used to evaluate the efficacy of the antibodies and vaccination.

Genomic mutations drive the evolution of all viruses. The mutations enable the virus to escape from immune response and acquire a selective advantage in transmission. A recent study from Los Alamos National Laboratory has identified a major mutation on SARS-CoV-2 Spike initially emerged in European countries. This D614G mutation has rapidly become dominant in these regions, suggesting this mutation may make the SARS-CoV-2 virus more transmissive. It's critical to understand the biology of this variant, particularly in the context of vaccine and drug design.
 
Sino Biological has developed a recombinant S1 protein with a D614G mutation (40591-V08H3). The recombinant 614G variant demonstrated an ACE2 binding activity.

In addition to the D614G mutation, several other mutations are also identified. Some of them are believed to enhance S1's ability to bind with ACE2. Sino Biological has developed a panel of mutated RBD proteins to facilitate the study of these variants.

SARS-CoV-2 Spike Mutant Protein

Cat# Description
40592-V08H80 SARS-CoV-2 Spike RBD-His tag (Y453F)
40591-V08H3 SARS-CoV-2 Spike S1-His tag (D614G)
40592-V08H46 SARS-CoV-2 Spike RBD-His tag (S477N)
40591-V02H3 SARS-CoV-2 (2019-nCoV) Spike S1(D614G), ECD-Fc Recombinant Protein
40589-V08H4 SARS-CoV-2 Spike S1+S2 ECD tag (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)
40592-V08H7 SARS-CoV-2 Spike RBD-His tag (K458R)
40592-V08H6 SARS-CoV-2 Spike RBD-His tag (F342L)
40592-V08H1 SARS-CoV-2 Spike RBD-His tag (V367F)
40592-V05H1 SARS-CoV-2 Spike RBD-mFc tag (V367F)
40592-V08H2 SARS-CoV-2 Spike RBD-His tag (N354D)
40592-V08H4 SARS-CoV-2 Spike RBD-His tag (A435S)
40592-V08H5 SARS-CoV-2 Spike RBD-His tag (V483A)
40592-V08H9 SARS-CoV-2 Spike RBD-His tag (W436R)
40592-V08H8 SARS-CoV-2 Spike RBD-His tag (G476S)
40592-V08H10 SARS-CoV-2 Spike RBD-His tag (R408I)
40592-V08H11 SARS-CoV-2 Spike RBD-His tag (V341I)
40592-V08H12 SARS-CoV-2 Spike RBD-His tag (Y508H)
40592-V08H14 SARS-CoV-2 Spike RBD-His tag (N439K)
40592-V08H15 SARS-CoV-2 Spike RBD-His tag (V503F)
40592-V08H16 SARS-CoV-2 Spike RBD-His tag (A522V)
40592-V08H18 SARS-CoV-2 Spike RBD-His tag (S494P)
40592-V08H19 SARS-CoV-2 Spike RBD-His tag (A372S)
40592-V08H20 SARS-CoV-2 Spike RBD-His tag (A520S)
40592-V08H21 SARS-CoV-2 Spike RBD-His tag (A522S)
40592-V08H22 SARS-CoV-2 Spike RBD-His tag (D405V, Q414A)
40592-V08H23 SARS-CoV-2 Spike RBD-His tag (Q414E)
40592-V08H24 SARS-CoV-2 Spike RBD-His tag (P384L)
40592-V08H25 SARS-CoV-2 Spike RBD-His tag (A348S)
40592-V08H26 SARS-CoV-2 Spike RBD-His tag (F338L)
40592-V08H27 SARS-CoV-2 Spike RBD-His tag (F377L)
40592-V08H28 SARS-CoV-2 Spike RBD-His tag (L452R)
40592-V08H29 SARS-CoV-2 Spike RBD-His tag (P521S)
40592-V08H30 SARS-CoV-2 Spike RBD-His tag (T478I)
40592-V08H31 SARS-CoV-2 Spike RBD-His tag (V483I)
40592-V08H32 SARS-CoV-2 Spike RBD-His tag (S359N)
40592-V08H33 SARS-CoV-2 Spike RBD-His tag (K378R)
40592-V08H34 SARS-CoV-2 Spike RBD-His tag (Q409E)
40592-V08H35 SARS-CoV-2 Spike RBD-His tag (I472V)
40592-V08H36 SARS-CoV-2 Spike RBD-His tag (A372T)
40592-V08H37 SARS-CoV-2 Spike RBD-His tag (A344S)
40592-V08H39 SARS-CoV-2 Spike RBD-His tag (A520V)
40592-V08H40 SARS-CoV-2 Spike RBD-His tag (E406Q)
40592-V08H41 SARS-CoV-2 Spike RBD-His tag (F490S)
40592-V08H42 SARS-CoV-2 Spike RBD-His tag (K378N)
40592-V08H43 SARS-CoV-2 Spike RBD-His tag (N370S)
40592-V08H44 SARS-CoV-2 Spike RBD-His tag (Q414R)
40592-V08H45 SARS-CoV-2 Spike RBD-His tag (S477I)
40592-V08H47 SARS-CoV-2 Spike RBD-His tag (T385A)
40592-V08H48 SARS-CoV-2 Spike RBD-His tag (T393P)
40592-V08H49 SARS-CoV-2 Spike RBD-His tag (V395I)
40592-V08H52 SARS-CoV-2 Spike RBD-His tag (G485S)
40592-V08H53 SARS-CoV-2 Spike RBD-His tag (G482S)
40592-V08H54 SARS-CoV-2 Spike RBD-His tag (K444R)
40592-V08H55 SARS-CoV-2 Spike RBD-His tag (N440K)
40592-V08H56 SARS-CoV-2 Spike RBD-His tag (E471Q)
40592-V08H57 SARS-CoV-2 Spike RBD-His tag (P479S)
40592-V08H58 SARS-CoV-2 Spike RBD-His tag (A352S)
40592-V08H59 SARS-CoV-2 Spike RBD-His tag (K417N)
40592-V08H62 SARS-CoV-2 Spike RBD-His tag (P337S)
40592-V08H63 SARS-CoV-2 Spike RBD-His tag (P521R)
40592-V08H64 SARS-CoV-2 Spike RBD-His tag (S477R)
40592-V08H51 SARS-CoV-2 Spike RBD-His tag (G446V)
40592-V08H69 SARS-CoV-3 Spike RBD-His tag (K458Q)
40592-V08H70 SARS-CoV-4 Spike RBD-His tag (N481D)
40592-V08H71 SARS-CoV-5 Spike RBD-His tag (F456L)
40592-V08H72 SARS-CoV-6 Spike RBD-His tag (Y505C)
40592-V08H73 SARS-CoV-7 Spike RBD-His tag (F456E)
40592-V08H74 SARS-CoV-8 Spike RBD-His tag (F486S)
40592-V08H76 SARS-CoV-9 Spike RBD-His tag (G446S)
40592-V08H78 SARS-CoV-10 Spike RBD-His tag (P499R)
40592-V08H79 SARS-CoV-11 Spike RBD-His tag (V445F)
Recombinant Antibody Production