SARS-CoV-2 Antibodies

The ongoing COVID-19 pandemics has sickened 4million people around the world, claiming over 280,000 lives. To address this global public health crisis, unprecedented efforts are being made to study the culprit virus, SARS-CoV-2. The biomedical community are working to develop rapid diagnostic reagents, vaccines, and therapies against this virus.

To support COVID-19 research, Sino Biological has developed a large collection of antibodies against different antigens of SARS-CoV-2. These antibodies can be used to detect and/or neutralize the virus in a variety of assays including ELISA, western blot, Immunofluorescent staining, Immunohistochemistry, SPR (Biacore and Octet), pseudovirus infection, and potentially in vivo animal studies.

SARS-CoV-2
ANTIBODY GUIDE
SARS-CoV-2
ANTIBODY GUIDE
SARS-CoV-2 Antibodies   SARS-CoV-2 Antigens  

Specific and Neutralizing Antibodies against SARS-CoV-2 Spike

Anti-SARS-CoV-2 S1 Neutralizing Antibody,
Mouse MAb (Catalog# 40591-MM43)

Anti-SARS-CoV-2 RBD Neutralizing Antibody,
Mouse MAb(Catalog# 40592-MM57)

Anti-SARS-CoV-2 RBD Neutralizing Antibody, Rabbit MAb(Catalog# 40592-R001)

Neutralization Activity Assay
(Measured by microneutralization assay in vitro)
The microneutralization assay was performed on 293T-ACE2 cells infected with SARS-CoV-2 Spike PSV under the treatment of serial dilutions of neutralizing antibodies. Neutralizing antibodies can effectively inhibit the infection of SARS-CoV-2 Spike pseudovirus on ACE2 overexpression 293T cells.
S1 Neutralizing Antibody
Conc. (µg/mL) Inhibition%
100 96.39%
10 94.57%
1 42.24%
0.1 -5.53%
The IC50 is typically 1.41 µg/mL.
RBD Neutralizing Antibody
Conc. (µg/mL) Inhibition%
100 95.51%
10 93.32%
1 75.69%
0.1 10.11%
The IC50 is typically 0.41 µg/mL.
RBD Neutralizing Antibody
Conc. (µg/mL) Inhibition%
100 99.56%
4 99.9%
0.16 59.29%
0.032 5.59%
The IC50 is typically 0.11 µg/mL.
 
Competitive-ELISA Assay
(Measured by Inhibitor Screening ELISA Kit )
Neutralizing ability was detected by SARS-CoV-2 Inhibitor Screening ELISA Kit (Catalog# KIT001) using competitive ELISA.
S1 Neutralizing Antibody
Mouse MAb

The IC50 is typically 0.857 nM
RBD Neutralizing Antibody
Mouse MAb

The IC50 is typically 3.694 nM
RBD Neutralizing Antibody
Rabbit MAb

The IC50 is typically 0.066 nM
Specificity Assay
SARS-CoV-2 shares an 80% sequence identity to the 2003 SARS-CoV. Hence, antibodies raised against SARS-CoV-2 antigens often shows a various degree of cross-reactivity with the corresponding antigens of 2003 SARS-CoV. The similarities between SARS-CoV-2 and other members of human coronavirus are very limited. These two neutralizing antibodies are specific to the SARS-CoV-2 which have no cross-reactivity with other 6 Human coronaviruses.
S1 Neutralizing Antibody
RBD Neutralizing Antibody
ELISA-(cross) reactivity was assessed of all 7 kinds of human coronavirus Spike S1 and RBD protein, including SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-HKU1, HCoV-229E, HCoV-OC43, HCoV-NL65. These two SARS-CoV-2 antibodies are specific to SARS-CoV-2 spike antigen, other human coronaviruses displayed no ELISA-cross-reactivity with them(OD450 in grey).
Affinity Assay
The binding between immobilized SARS-CoV-2 RBD protein (40592-V08B-B) and the anti-Spike antibody 40592-R001 in Octet RED assay.

Antibody Against SARS-CoV-2 Spike S2

This chimeric antibody specifically recognizing the S2 domain of SARS-CoV-2

Cat # 40590-D001
Description SARS-CoV-2 Spike S2 Antibody
Type Chimeric MAb
Ig Type mouse (variable region) / human (kappa / IgG1 constant)
Cross-reactivity No cross-reactivity in ELISA with
SARS-CoV-2 (2019-nCoV) Spike S1 (Cat# 40591-V08H)
SARS-CoV-2 (2019-nCoV) Spike RBD (Cat# 40592-V05H)

Monoclonal Antibody Pairs for Sandwich ELISA-based Detection of SARS-CoV-2

 
 
N-Antibody
40143-MM05 : 40143-R001
40143-R004 : 40143-R040
 
S-Antibody
40150-D001 : 40150-D003
40150-D002 : 40150-D004
2019-nCoV Spike Detection Kit
(KIT40591)
They can be use to detection or diagnostic tools development. Sino Biological has released the world-first ELISA kits for the detection of SARS-CoV-2 N and S proteins. These assays may be used to quantify antigens on the surface of SARS-CoV-2 and/or pseudovirus , as well as antigens expressed recombinantly.
Validated Applications
IF (40150-R007) - Pseudovirus Validated

SARS-CoV-2 Spike in ACE2-overexpressed 293T cells, infected (left) or noninfected (right) by 2019-nCOV pseudovirus.
WB (40143-R019)

Sample: 2019-nCoV NP Protein (Cat# 40588-V08B)
Lane A: 30ng
Lane B: 10ng
Lane C: 5ng
Lane D: 2ng
Lane E: 1ng
Lane F: 0.5ng

SARS-CoV-2 Antibody List

Cat#   Antigen More
40588-T62   Nucleocapsid

Description SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody
Type Rabbit PAb
40143-R001   Nucleocapsid

Description SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody
Type Rabbit MAb
40143-MM05   Nucleocapsid

Description SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody
Type Mouse MAb
40143-R019   Nucleocapsid

Description SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody
Type Rabbit MAb
40143-MM08   Nucleocapsid

Description SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody
Type Mouse MAb
40143-R040   Nucleocapsid

Description SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody
Type Rabbit MAb
40143-R004   Nucleocapsid

Description SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody
Type Rabbit MAb
40588-R0004   Nucleocapsid

Description SARS-CoV-2 Nucleocapsid Antibody
Type Rabbit MAb
40150-R007   Spike

Description SARS-CoV-2 (2019-nCoV) Spike S1 Antibody
Type Rabbit MAb
40591-MM42   Spike

Description SARS-CoV-2 (SARS-CoV-2) Spike S1 Antibody
Type Mouse MAb
40150-D006   Spike

Description SARS-CoV/SARS-CoV-2 Spike Antibody
Type Chimeric MAb
40150-D004   Spike

Description SARS-CoV/SARS-CoV-2 Spike antibody
Type Chimeric MAb
40150-D00   Spike

Description SARS-CoV/SARS-CoV-2 Spike antibody
Type Chimeric MAb
40588-T62   Spike

Description SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody
Type Rabbit PAb
40150-D002   Spike

Description SARS-CoV/SARS-CoV-2 Spike antibody
Type Chimeric MAb
40150-D001   Spike

Description SARS-CoV/SARS-CoV-2 Spike antibody
Type Chimeric MAb
40592-T62   Spike

Description SARS-CoV-2 (2019-nCoV) Spike RBD Antibody
Type Rabbit PAb
40590-T62   Spike

Description SARS-CoV-2 (2019-nCoV) Spike S2 Antibody
Type Rabbit PAb
40590-D001   Spike

Description SARS-CoV-2 (2019-nCoV) Spike S2 Antibody
Type Chimeric MAb
40591-MM43   Spike

Description SARS-CoV-2 Spike S1 Neutralizing Antibody
Type Mouse MAb
40592-MM57   Spike

Description SARS-CoV-2 Spike RBD Neutralizing Antibody
Type Mouse MAb
40592-R001   Spike

Description SARS-CoV-2 Spike RBD Neutralizing Antibody
Type Rabbit MAb
40591-T62   Spike

Description SARS-CoV-2 Spike S1 Antibody
Type Rabbit PAb
40589-T62   Spike

Description SARS-CoV-2 (2019-nCoV) Spike Antibody
Type Rabbit PAb
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