Cluster of differentiation in B lymphocyte

Clusters of Differentiation (CD) proteins are a group of cell surface markers that can be used to identify different stages of B cell development or activation, including progenitor B cells, pro-B cells, pre-B cells, immature B cells, transitional 1 B cells, transitional 2 B cells, marginal zone B cells, na?ve mature follicular B cells, activated germinal center B cells, memory B cells, plasmablast cells, and plasma cells. Other cell surface markers used to identify specific stages of B cell development or activation are also listed.

For example, CD138 distinguishes circulating plasmablasts and plasma cells, from other developmental and functional subsets. The key marker for B-cell panels is the lineage marker CD19, which is expressed by almost all cells belonging to the B-cell lineage. In the mouse, CD45R/B220 is traditionally used. Depending on the type of sample that is being analyzed, different markers can be selected to define the specific B-cell subsets of interest. The example shown analyzes human peripheral blood using CD19, CD20, IgD, CD27, CD38, and CD24. Adding activation markers (CD69, CD25, CD80, CD86) can inform about which subsets are activated, and provide important clues about an individual's immune response.

Summary of the key developmental stages and CD antigens of B cells

Human positive markers CD10, CD34, CD38 CD10, CD19, CD24, CD34, CD38, CD123, CD127 CD10, CD19, CD20, CD24, CD38, CD123, CD124, CD127 CD10, CD19, CD20, CD21, CD24, CD38, CD40, CD124
Human negative markers CD117 CD117 CD34, CD117 CD27, CD117, CD127
Mouse positive markers CD43, CD45, CD93, CD127, CD135, CD184 CD19, CD24, CD43, CD45, CD127 CD19, CD24, CD25, CD45,  CD127 CD19, CD24, CD45, CD93
Mouse negative markers CD19, CD24, CD117 CD117, CD135 CD43, CD117 CD23, CD43
  Memory B Activated B Follicular B Transitional B
Human positive markers CD19, CD20, CD27, CD40, CD184, CD185 CD19, CD20, CD25, CD27, CD30, CD69, CD80, CD86, CD135 CD19, CD20, CD21, CD22, CD23, CD24 CD5, CD19, CD20, CD21, CD24, CD38
Human negative markers CD23, CD38   CD10, CD24, CD27, CD38 CD10,CD27
Mouse positive markers CD38, CD45, CD62L, CD80, CD95 CD27, CD45, CD69, CD80, CD135   CD19, CD21, CD23, CD24, CD45, CD93
Mouse negative markers CD138, CD184