Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 protein (His Tag)

Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 protein (His Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
活性
Testing in progress
タンパク質の構築
A DNA sequence encoding the Zika virus (strain Zika SPH2015) NS1 (ALU33341.1) (Val796-Leu1157) was expressed with a polyhistidine tag at the N-terminus.
NP No.
発現ホスト
HEK293 Cells
ZIKV
予測N末端
His
分子量
The recombinant Zika virus (strain Zika SPH2015) NS1 consists of 381 amino acids and predicts a molecular mass of 43.5 kDa. The apparent molecular mass of the protein is approximately 50 kDa in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 protein (His Tag): 画像

ZIKV NS-1 protein 背景情報

Zika virus NS1 antigen is one of seven non-structural proteins. NS1 is involved in RNA replication. The possible effects of NS1 on hosts include: localizes to host cell surface and secreted extracellularly, modulates signalling of the innate immune system, has possible damages to platelets and endothelial cells through anti-NS1 antibodies.
参考文献
  • Wong SS-Y, et al., Zika virus infectiondthe next wave after dengue?, Journal of the Formosan Medical Association (2016)
  • Deciphering Protein O-Glycosylation: Solid-Phase Chemoenzymatic Cleavage and Enrichment
    Author
    Yang, S;Onigman, P;Wu, WW;Sjogren, J;Nyhlen, H;Shen, RF;Cipollo, J;
    Year
    2018
    Journal
    Anal. Chem.
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