Zika virus (ZIKV) (strain Zika SPH2015) prME protein ( His Tag)

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Zika virus (ZIKV) (strain Zika SPH2015) prME protein ( His Tag): 製品情報

純度
> 90 % as determined by SDS-PAGE.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
活性
Testing in progress
タンパク質の構築
A DNA sequence encoding the Zika virus (strain Zika SPH2015) prME (ALU33341.1) (Phe257-Gly694) was expressed with two additional amino acids (Met & Val) at the N-terminus and a polyhistidine tag at the C-terminus.
NP No.
発現ホスト
Baculovirus-Insect Cells
ZIKV
予測N末端
Met
分子量
The recombinant Zika virus (strain Zika SPH2015) prME consists of 415 amino acids and predicts a molecular mass of 49.3 kDa.
バッファー
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Zika virus (ZIKV) (strain Zika SPH2015) prME protein ( His Tag): 画像

Citation List

ZIKV Envelope 背景情報

Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
参考文献
  • Marina Basarab specialty registrar. et al .Zika virus. BMJ 2016; 352:i1049 doi: 10.1136/bmj.i1049
  • Pinto Júnior VL, et al. Zika virus: a review to clinicians, Acta Med Port 2015 Nov-Dec;28(6):760-765
  • L Zammarchi, et al.Zika virus infection in a traveler returning to Europe from Brazil, March 2015
  • Arturo Galindo-Fraga, et al. Zika virus: A new epidemic on our doorstep. Rev Inves Clin.2015, 67: 329-32
  • Convalescent patient-derived monoclonal antibodies targeting different epitopes of E protein confer protection against Zika virus in a neonatal mouse model
    Author
    Niu, X;Zhao, L;Qu, L;Yao, Z;Zhang, F;Yan, Q;Zhang, S;Liang, R;Chen, P;Luo, J;Xu, W;Lv, H;Liu, X;Lei, H;Yi, C;Li, P;Wang, Q;Wang, Y;Yu, L;Zhang, X;Bryan, LA;Davidson, E;Doranz, JB;Feng, L;Pan, W;Zhang, F;Chen, L;
    Year
    2019
    Journal
    Emerg Microbes Infect
    Application
    cell sorting; ELISA and octet
  • Longitudinal analysis of the antibody repertoire of a Zika virus-infected patient revealed dynamic changes in antibody response
    Author
    Niu, X;Yan, Q;Yao, Z;Zhang, F;Qu, L;Wang, C;Wang, C;Lei, H;Chen, C;Liang, R;Luo, J;Wang, Q;Zhao, L;Zhang, Y;Luo, K;Wang, L;Wu, H;Liu, T;Li, P;Zheng, Z;Tan, YJ;Feng, L;Zhang, Z;Han, J;Zhang, F;Chen, L;
    Year
    2020
    Journal
    Emerg Microbes Infect
    Application
    in vitro binding assay
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