SuperNuclease
Cat: GMP-SSNP01
Cat: GMP-SSNP01
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General Information
| Manufactured By: | Sino Biological Inc. |
| Synonym: | Nuclease, Serratia marcescens’ extracellular endonuclease |
| Protein Construction: | SuperNuclease is a recombined Serratia marcescens’ extracellular endonuclease. |
| Source: | Aequorea victoria |
| Expression Host: | Escherichia coli (E. coli). |
QC Testing
| Purity: | > 95 % as determined by SDS-PAGE. > 99 % as determined by SEC-HPLC Analysis. |
| Endotoxin: | < 0.01 EU/1000 units as determined by the LAL method. |
| Activity: | Measured by its ability to cleave Salmon sperm DNA substrate (Sigma, Catalog # D1626). One unit is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 minutes at 37°C. The specific activity is ≥ 1.1×106 units/mg. |
| Stability: | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. |
| Predicted N terminal: | Three isoforms with different N terminal may be found from the compound—Sm1 (22D-266N), Sm2 (23T-266N) and Sm3 (25E-266N), the activity analysis shows that they were functionally equivalent. |
| Molecular Mass: | The SuperNuclease comprises 266 amino acids and has a calculated molecular mass of Sm1 (26708.2 Da), Sm2 (26591.8 Da) and Sm3 (26376.4 Da). The apparent molecular mass of SuperNuclease is approximately 26.5 Kda. |
SDS-PAGE:
SEC-HPLC:
Usage Guide
| Storage: | Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. |
Protocol
Large scale cell lysis treatment:
1). Lysis buffer preparation:
The lysis buffer should be suitable for the protein as well as the downstream purification processes, and so on;
2). Resuspend cell plates in lysis buffer:
The ratio of lysis buffer (mL) against the gram of cell can be (10-20):1;
3). Add SuperNuclease (250 units to 1 g cells):
It is well recommended to optimize the amount of SuperNuclease;
4). Lysate cell by mechanical or chemical methods on ice or at room temperature.
Methods: Ultrasonic disruption, High Pressure Homogenizer, tissue homogenizer, and so on;
5). Obtain clear cell lysate supernatant by centrifugation at ~12,000 rpm for 0.5 hour.
