HER3/ERBB3 Protein, Rhesus, Recombinant
HER3/ERBB3 Protein, Rhesus, Recombinant: 製品情報
純度
> 90 % as determined by SDS-PAGE.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
活性
1. Measured by its binding ability in a functional ELISA.
2. Immobilized Rhesus ERBB3 at 10 μg/mL (100 μL/well) can bind Human Fc-NRG1 (isoform Beta1) (Cat:11609-H01H). The EC50 of Human Fc-NRG1 (isoform Beta1) is 0.51-1.19 μg/mL.
2. Immobilized Rhesus ERBB3 at 10 μg/mL (100 μL/well) can bind Human Fc-NRG1 (isoform Beta1) (Cat:11609-H01H). The EC50 of Human Fc-NRG1 (isoform Beta1) is 0.51-1.19 μg/mL.
タンパク質の構築
A DNA sequence encoding the rhesus ERBB3 (XP_001113953.2) (Met1-Thr643) was expressed with six amino acids (LEVLFQ) at the C-terminus.
NP No.
発現ホスト
HEK293 Cells
種
Rhesus
予測N末端
Ser 20
分子量
The recombinant rhesus ERBB3 consists 631 amino acids and predicts a molecular mass of 69.5 kDa.
バッファー
Lyophilized from sterile PBS, 10 % Glycerol, 0.01 % Tween 20, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
HER3/ERBB3 Protein, Rhesus, Recombinant: 画像
HER3/ERBB3 背景情報
ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
完全な名称
erb-b2 receptor tyrosine kinase 3
参考文献
- Kraus M.H., et al.,(1989), Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc. Natl. Acad. Sci. U.S.A. 86:9193-9197.
- Plowman G.D., et al., (1990), Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909.
- Katoh M., et al.,(1993), c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.Biochem. Biophys. Res. Commun. 192:1189-1197.
Human HER3/ERBB3 タンパク質
Mouse HER3/ERBB3 タンパク質
If you couldn't find the specific citation, you can:
