TGF beta 1 Protein, Rat/Mouse, Recombinant

TGF beta 1 Protein, Rat/Mouse, Recombinant: 製品情報

純度
> 90 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50 for this effect is 0.2-0.8 ng/mL.
タンパク質の構築
A DNA sequence encoding the mouse / rat TGFB1 (Ala279-Ser390) was expressed. Rat and Mouse mature TGFB1 sequences are identical.
発現ホスト
HEK293 Cells
Mouse, Rat
予測N末端
Ala 279
分子量
The recombinant mouse / rat TGFB1 comprises 112 amino acids and predicts a molecular mass of 12.8 kDa.
バッファー
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TGF beta 1 Protein, Rat/Mouse, Recombinant: 画像

Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50 for this effect is 0.2-0.8 ng/mL.

TGF beta 1 背景情報

TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-beta 1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta 1 also has the opposite effect on cells of myeloid origin. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.
完全な名称
transforming growth factor, beta 1
Related Pathways
  • P38 MAPK Signaling Pathway
    P38 MAPK Signaling Pathway
参考文献
  • Ghadami M, et al. (2000) Genetic Mapping of the Camurati-Engelmann Disease Locus to Chromosome 19q13.1-q13.3. Am J Hum. Genet. 66(1):143-7.
  • Letterio J, et al. (1998) Regulation of immune responses by TGF-beta. Annu Rev Immunol. 16:137-61.
  • Vaughn SP, et al. (2000) Confirmation of the mapping of the Camurati-Englemann locus to 19q13. 2 and refinement to a 3.2-cM region. Genomics. 66(1):119-21.
  • Assoian R, et al. (1983) Transforming growth factor-beta in human platelets. Identification of a major storage site, purification, and characterization. J Biol Chem. 258(11):7155-60.
  • Deficient O-GlcNAc Glycosylation Impairs Regulatory T Cell Differentiation and Notch Signaling in Autoimmune Hepatitis
    Author
    Hao, X;Li, Y;Wang, J;Ma, J;Zhao, S;Ye, X;He, L;Yang, J;Gao, M;Xiao, F;Wei, H;
    Year
    2018
    Journal
    Front Immunol
    Application
    cell-based (Treg)
  • Inhibition of TGF-β induced lipid droplets switches M2 macrophages to M1 phenotype
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    Bose, D;Banerjee, S;Chatterjee, N;Das, S;Saha, M;Saha, KD;
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    2019
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    Toxicol In Vitro
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    cell-based
  • CTGF Triggers Rat Astrocyte Activation and Astrocyte-Mediated Inflammatory Response in Culture Conditions
    Author
    Lu, M;Yan, XF;Si, Y;Chen, XZ;
    Year
    2019
    Journal
    Inflammation
    Application
    cell-based
  • CircPlekha7 plays an anti-fibrotic role in intrauterine adhesions by modulating endometrial stromal cell proliferation and apoptosis
    Author
    Xie, W;He, M;Liu, Y;Huang, X;Song, D;Xiao, Y;
    Year
    2020
    Journal
    J. Reprod. Dev.
    Application
    cell-based
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