Vitronectin Protein, Mouse, Recombinant (His Tag)

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Vitronectin Protein, Mouse, Recombinant (His Tag): 製品情報

純度
> 85 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells.
When cells are added to mouse Vitronectin coated plates (10 μg/mL and 100 μL/well), > 60% cells will adhere specifically after 30 minutes at 37 ℃.
タンパク質の構築
A DNA sequence encoding the mouse vitronectin (NP_035837.1) (Met 1-Lys 478) was expressed, with a polyhistidine tag at the C-terminus.
NP No.
発現ホスト
HEK293 Cells
Mouse
予測N末端
Asp 20
分子量
The secreted recombinant mouse vitronectin consists of 470 amino acids and has a predicted molecular mass of 54.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmvitronectin is approximately 75-85 kDa due to glycosylation.
バッファー
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Vitronectin Protein, Mouse, Recombinant (His Tag): 画像

Vitronectin Protein, Mouse, Recombinant (His Tag): 別名

AI256434 Protein, Mouse; Vn Protein, Mouse

Vitronectin 背景情報

Vitronectin, also known as VTN, is a member of the pexin family. It is an abundant glycoprotein found in serum the extracellular matrix and promotes cell adhesion and spreading. Vitronectin is a secreted protein and exists in either a single chain form or a cleaved, two chain form held together by a disulfide bond. Vitronectin is a plasma glycoprotein implicated as a regulator of diverse physiological process, including blood coagulation, fibrinolysis, pericellular proteolysis, complement dependent immune responses, and cell attachment and spreading. Because of its ability to bind platelet glycoproteins and mediate platelet adhesion and aggregation at sites of vascular injury, vitronectin has become an important mediator in the pathogenesis of coronary atherosclerosis. As a multifunctional protein with a multiple binding domain, Vitronectin interacts with a variety of plasma and cell proteins. Vitronectin binds multiple ligands, including the soluble vitronectin receptor. It may be an independent predictor of adverse cardiovascular outcomes following acute stenting. Accordingly, Vitronectin is suggested to be involved in hemostasis, cell migration, as well as tumor malignancy.
完全な名称
vitronectin
参考文献
  • Ekmeki OB, et al. (2006) Vitronectin in atherosclerotic disease. Clin Chim Acta. 368(1-2): 77-83.
  • Derer W, et al. (2009) Vitronectin concentrations predict risk in patients undergoing coronary stenting. Circ Cardiovasc Interv. 2(1): 14-9.
  • Heyman L, et al. (2010) Mesothelial vitronectin stimulates migration of ovarian cancer cells. Cell Biol Int. 34(5): 493-502.
  • Vitronectin promotes the vascularization of porous polyethylene biomaterials
    Author
    Hessenauer, MET;Lauber, K;Zuchtriegel, G;Uhl, B;Hussain, T;Canis, M;Strieth, S;Berghaus, A;Reichel, CA;
    Year
    2018
    Journal
    Acta Biomater
    Application
    animal-based
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