P-Selectin Protein, Mouse, Recombinant (His Tag)

P-Selectin Protein, Mouse, Recombinant (His Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by the ability of the immobilized protein to support the adhesion of U937 cells.When 5 x 10E4 cells/well are added to SELP-coated plates (5 μg/mL and 100 μL/well), approximately >60% cells will adhere specifically after 60 minutes at 37℃.
タンパク質の構築
A DNA sequence encoding the mouse SELP (Q01102) (Met1-Ala709) was expressed with a C-terminal polyhistidine tag.
Uniprot No.
発現ホスト
HEK293 Cells
Mouse
予測N末端
Trp 42
分子量
The recombinant mouse SELP comprises 679 amino acids and has a predicted molecular mass of 74 kDa. The apparent molecular mass of the protein is approximately 116 kDa in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

P-Selectin Protein, Mouse, Recombinant (His Tag): 画像

P-Selectin Protein, Mouse, Recombinant (His Tag): 別名

CD62P Protein, Mouse; GMP-140 Protein, Mouse; Grmp Protein, Mouse; LECAM3 Protein, Mouse; PADGEM Protein, Mouse

P-Selectin 背景情報

P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes.
完全な名称
selectin P (granule membrane protein 140kDa, antigen CD62)
参考文献
  • Johnson-Tidey RR, et al. (1994) Increase in the adhesion molecule P-selectin in endothelium overlying atherosclerotic plaques. Coexpression with intercellular adhesion molecule-1. Am J Pathol. 144(5):952-61.
  • Walcheck B, et al. (1996) Neutrophil-neutrophil interactions under hydrodynamic shear stress involve L-selectin and PSGL-1. A mechanism that amplifies initial leukocyte accumulation of P-selectin in vitro. J Clin Invest. 98(5):1081-7.
  • Foreman KE, et al. (1994) C5a-induced expression of P-selectin in endothelial cells. J Clin Invest. 94(3):1147-55.
  • Chrono-pharmacological Targeting of the CCL2-CCR2 Axis Ameliorates Atherosclerosis
    Author
    Winter, C;Silvestre-Roig, C;Ortega-Gomez, A;Lemnitzer, P;Poelman, H;Schumski, A;Winter, J;Drechsler, M;de Jong, R;Immler, R;Sperandio, M;Hristov, M;Zeller, T;Nicolaes, GAF;Weber, C;Viola, JR;Hidalgo, A;Scheiermann, C;Soehnlein, O;
    Year
    2018
    Journal
    Cell Metab.
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