Vimentin Protein, Human, Recombinant (His Tag)

Vimentin Protein, Human, Recombinant (His Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Testing in progress
タンパク質の構築
A DNA sequence encoding the human VIM (Met1-Glu466) (P08670) was expressed with a C-terminal polyhistidine tag.
Uniprot No.
発現ホスト
Baculovirus-Insect Cells
Human
予測N末端
Met 1
分子量
The secreted recombinant human VIM consists of 477 amino acids and predicts a molecular mass of 55.11 KDa.
バッファー
Lyophilized from sterile 40% acetonitrile, 0.1% TFA
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Vimentin Protein, Human, Recombinant (His Tag): 画像

Vimentin Protein, Human, Recombinant (His Tag): 別名

CTRCT30 Protein, Human; HEL113 Protein, Human

Vimentin 背景情報

Vimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl (tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme.
完全な名称
vimentin
参考文献
  • Russell RL, et al. (2001) Uridine phosphorylase association with vimentin. Intracellular distribution and localization. J Biol Chem. 276(16):13302-7.
  • Moinova, et al. (2012) Aberrant Vimentin Methylation is Characteristic of Upper GI Pathologies. Cancer Epidemiology Biomarkers Prev. 21(4):594-600.
  • Leader M, et al. (1987) Vimentin: an evaluation of its role as a tumour marker. Histopathology. 11(1):63-72.
  • Extracellular vimentin modulates human dendritic cell activation
    Author
    Yu, MB;Guerra, J;Firek, A;Langridge, WHR;
    Year
    2018
    Journal
    Mol. Immunol.
    Application
    cell-based (DC activation)
  • Click RNA for Rapid Capture and Identification of Intracellular MicroRNA Targets
    Author
    Zhang, P;Fu, H;Du, S;Wang, F;Yang, J;Cai, W;Liu, D;
    Year
    2019
    Journal
    Anal. Chem.
    Application
    binding
  • Vimentin-targeting AIEgen-peptide conjugates: Wash-free fluorescence detection of EMT-type cancer cells and tissues
    Author
    Wei, H;Yu, Y;Wang, Y;Ying, Z;Cheng, W;Tian, X;Kan, Q;
    Year
    2020
    Journal
    Sensors and Actuators B: Chemical
    Application
    fluorescence titration; confocal
  • Artemisinins target the intermediate filament protein vimentin for human cytomegalovirus inhibition
    Author
    Roy, S;Kapoor, A;Zhu, F;Mukhopadhyay, R;Ghosh, AK;Lee, H;Mazzone, J;Posner, GH;Arav-Boger, R;
    Year
    2020
    Journal
    J. Biol. Chem.
    Application
    substrate
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