TIM-3 Protein, Human, Recombinant (ECD, His Tag)

TIM-3 Protein, Human, Recombinant (ECD, His Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
活性
Labeled biotin to TIM-3 Protein, Human, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of TIM-3 Protein, Human, Recombinant (His Tag), Biotinylated (Cat. 10390-H08H-B) bound to Anti-TIMD3 Antibody was 0.1 nM.
タンパク質の構築
A DNA sequence encoding the human HAVCR2 ( AAL65157.1) (Met1-Arg200) was expressed with a polyhistidine tag at the C-terminus.
Uniprot No.
発現ホスト
HEK293 Cells
Human
予測N末端
Ser 22
分子量
The recombinant human HAVCR2 consists of 190 amino acids and predicts a molecular mass of 21.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human HAVCR2 is approximately 42.8 kDa due to glycosylation.
バッファー
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TIM-3 Protein, Human, Recombinant (ECD, His Tag): 画像

Labeled biotin to TIM-3 Protein, Human, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of TIM-3 Protein, Human, Recombinant (His Tag), Biotinylated (Cat. 10390-H08H-B) bound to Anti-TIMD3 Antibody was 0.1 nM.

TIM-3 Protein, Human, Recombinant (ECD, His Tag): 別名

CD366 Protein, Human; HAVcr-2 Protein, Human; HAVCR2 Protein, Human; KIM-3 Protein, Human; Tim-3 Protein, Human; TIM3 Protein, Human; TIMD-3 Protein, Human; TIMD3 Protein, Human

TIM-3 背景情報

Hepatitis A virus cellular receptor 2 (HAVCR2), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. It was the first surface molecule that specifically identifies Th1 cells in both the mouse and human. Recently, the identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Engagement of Tim-3 by its ligand galectin-9 negatively regulates IFN-gamma secretion and influences the ability to induce T cell tolerance in both mice and man. It suggests a novel paradigm in which dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states, such as multiple sclerosis. Recent work has explored the role of TIM-3 in systemic lupus erythematosus (SLE), and their results indicate that TIM-3 may represent a novel target for the treatment of SLE. Numerous studies have demonstrated that Tim-3 influences autoimmune diseases, including diabetes and multiple sclerosis, and its role in other inflammatory diseases including allergies and cancer is beginning to become clear. In the tumor rejection model, the soluble form of Tim-3 (sTim-3) significantly impaired T cell antitumor immunity, evidenced by decreased antitumor CTL activity and reduced amount of tumor-infiltrating lymphocytes in the tumor. sTim-3 as an immunoregulatory molecule that may be involved in the negative regulation of T cell-mediated immune response.
完全な名称
hepatitis A virus cellular receptor 2
研究分野
参考文献
  • Geng H, et al. (2006) Soluble form of T cell Ig mucin 3 is an inhibitory molecule in T cell-mediated immune response. J Immunol. 176(3): 1411-20.
  • Anderson AC, et al. (2006) TIM-3 in autoimmunity. Curr Opin Immunol. 18(6): 665-9.
  • Anderson DE. (2007) TIM-3 as a therapeutic target in human inflammatory diseases. Expert Opin Ther Targets. 11(8): 1005-9.
  • Pan HF, et al. (2010) TIM-3 as a new therapeutic target in systemic lupus erythematosus. Mol Biol Rep. 37(1): 395-8.
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