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PD-L2 Protein, Human, Recombinant (His Tag)

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PD-L2 Protein, Human, Recombinant (His Tag): 製品情報

純度
> 98 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by its binding ability in a functional ELISA . Immobilized recombinant human PD-L2 at 1 μg/ml (100 μl/well) can bind recombinant human PD1 with a linear range of 7.8-1000 ng/ml .
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met 1-Pro 219) of human PD-L2 (NP_079515.2) was expressed with a C-terminal polyhistidine tag.
NP No.
発現ホスト
HEK293 Cells
Human
予測N末端
Leu 20
分子量
The recombinant human PD-L2 consists of 211 amino acids and predicts a molecular mass of 24 kDa. As a result of glycosylation, the apparent molecular mass of rh PD-L2 is approximately 40-45 kDa in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PD-L2 Protein, Human, Recombinant (His Tag): 画像

Measured by its binding ability in a functional ELISA . Immobilized recombinant human PD-L2 at 1 μg/ml (100 μl/well) can bind recombinant human PD1 with a linear range of 7.8-1000 ng/ml

PD-L2 Protein, Human, Recombinant (His Tag): 別名

B7-DC Protein, Human; B7DC Protein, Human; bA574F11.2 Protein, Human; Btdc Protein, Human; CD273 Protein, Human; PD-L2 Protein, Human; PDCD1L2 Protein, Human; PDL2 Protein, Human

PD-L2 背景情報

Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-&gamma; in monocytes, or LPS plus IFN-&gamma; in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.
完全な名称
programmed cell death 1 ligand 2
参考文献
  • Latchman Y, et al. (2001) PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2: 261-8.
  • Carreno BM, et al. (2005) Therapeutic opportunities in the B7/CD28 family of ligands and receptors. Curr Opin Pharmacol. 5(4): 424-30.
  • Radhakrishnan S, et al. (2007) B7-DC/PD-L2 cross-linking induces NF-kappaB-dependent protection of dendritic cells from cell death. J Immunol. 178(3): 1426-32.
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