< 1.0 EU per μg protein as determined by the LAL method.
Measured by its binding ability in a functional ELISA. Immobilized recombinant Human TNFRSF4-His (Cat:10481-H08H) at 10 μg/ml (100 μl/well) can bind human S4-mFc3-TNFSF4 (Cat:13127-H04H）with a linear range of 1.28-20 μg/ml.
A DNA sequence encoding the human TNFSF4 (NP_003317.1) (Gln51-Leu183) was expressed with the Fc region of mouse IgG1 at the N-terminus.
The recombinant human TNFSF4 consists of 369 amino acids and predicts a molecular mass of 42.1 kDa. As a result of glycosylation, it migrates as an approximately 53.3 kDa band in SDS-PAGE under reducing conditions.
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Measured by its binding ability in a functional ELISA.
Immobilized recombinant Human TNFRSF4-His (Cat:10481-H08H) at 10 μg/ml (100 μl/well) can bind human S4-mFc3-TNFSF4 (Cat:13127-H04H）with a linear range of 1.28-20 μg/ml.
OX-40L, also known as TNFSF4 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. OX-40L is an important costimulatory molecule that plays a crucial role in the regulation of T-cell-mediated immunity. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. OX-40L is a ligand for receptor TNFRSF4/OX40. It is found to play a role in T cell antigen-presenting cell (APC) interactions. In surface Ig- and CD4-stimulated B cells, this cytokine along with CD7 has been shown to provide CD28-independent costimulatory signals to T cells. This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells.