Nectin-2 Protein, Human, Recombinant (hFc Tag)

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Nectin-2 Protein, Human, Recombinant (hFc Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by its binding ability in a functional ELISA . Immobilized human CD226 at 2 μg/ml (100 μl/well) can bind human CD112 with a linear ranger of 6.4-800 ng/ml
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met 1-Leu 360) of human CD112 (NP_002847.1) was fused with the Fc region of human IgG1 at the C-terminus.
NP No.
発現ホスト
HEK293 Cells
Human
予測N末端
Gln 32
分子量
The recombinant mature human CD112/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 567 amino acids and has a calculated molecular mass of 62.3 kDa. As a result of glycosylation, the rh CD112/Fc monomer migrates as an approximately 70-80 kDa protein in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Nectin-2 Protein, Human, Recombinant (hFc Tag): 画像

Measured by its binding ability in a functional ELISA. Immobilized human CD226 at 2 μg/ml (100 μl/well) can bind human CD112 with a linear ranger of 6.4-800 ng/ml

Nectin-2 Protein, Human, Recombinant (hFc Tag): 別名

CD112 Protein, Human; HVEB Protein, Human; Nectin-2 Protein, Human; PRR2 Protein, Human; PVRR2 Protein, Human

Nectin-2 背景情報

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
完全な名称
poliovirus receptor-related 2 (herpesvirus entry mediator B)
参考文献
  • Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.
  • Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
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