IL13RA2 Protein, Human, Recombinant (ECD, His Tag)

IL13RA2 Protein, Human, Recombinant (ECD, His Tag): 製品情報

純度
> 85 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
活性
Measured by its ability to inhibit IL13-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.02-0.08 μg/mL.
タンパク質の構築
A DNA sequence encoding the extracellular domain of human IL13Rα2 (NP_000631.1) (Met1-Leu342) was expressed with the a polyhistidine tag at the C-terminus.
NP No.
発現ホスト
HEK293 Cells
Human
予測N末端
Asp 27
分子量
The recombinant human IL13Rα2 consists of 325 amino acids and predicts a molecular mass of 38 KDa. As a result of glycosylation, it migrates as an approximately 49.7 kDa band in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL13RA2 Protein, Human, Recombinant (ECD, His Tag): 画像

IL13RA2 Protein, Human, Recombinant (ECD, His Tag): 別名

CD213A2 Protein, Human; CT19 Protein, Human; IL-13R Protein, Human; IL13BP Protein, Human

IL13RA2 背景情報

Interleukin-13 receptor subunit alpha-2 (IL13RA2/IL-13RA2) is also known as cluster of differentiation 213A2 (CD213A2), IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, and IL-13RA2. The IL13RA2 is often overexpressed in brain tumors, making Il13ra2 one of the vaccine targets for immunotherapy of glioma. IL13RA2/IL-13RA2 is a cancer-associated receptor that is present in greater than 80% of High-Grade Astrocytomas (HGA) and has recently been recognized as a cytokine that predisposes breast cancer cells to metastasize. Expression of IL13Rα2 was rapidly lost from the surface of transduced cells grown in culture. The loss appeared to be related to ligands present in fetal bovine serum in the medium. None of the malignant glioma cell lines cultivated in vitro and tested to date exhibited the IL13Rα2 receptor. A recombinant virus (R5111) enters cells via its interaction with the IL13Rα2 receptor in a manner that cannot be differentiated from the interaction of wild-type virus with its receptors.
完全な名称
interleukin 13 receptor, alpha 2
参考文献
  • Zhou G, et al.. (2005) Characterization of a recombinant herpes simplex virus 1 designed to enter cells via the IL13Ralpha2 receptor of malignant glioma cells. J Virol. 79(9): 5272-7.
  • Osawa M, et al.. (2000) Characterization of the mouse interleukin-13 receptor alpha1 gene. Immunogenetics. 51(11): 974-81.
  • Nair BG, et al.. (2011) Nanotechnology platforms; an innovative approach to brain tumor therapy. Med Chem. 7(5): 488-503.
  • Benson M, et al.. (2006) A network-based analysis of the late-phase reaction of the skin. J Allergy Clin Immunol. 118(1): 220-5.
  • Chitinase 3-like 1-CD44 interaction promotes metastasis and epithelial-to-mesenchymal transition through β-catenin/Erk/Akt signaling in gastric cancer
    Author
    Geng, B;Pan, J;Zhao, T;Ji, J;Zhang, C;Che, Y;Yang, J;Shi, H;Li, J;Zhou, H;Mu, X;Xu, C;Wang, C;Xu, Y;Liu, Z;Wen, H;You, Q;
    Year
    2018
    Journal
    J. Exp. Clin. Cancer Res.
  • Drug Conjugates for Targeting Eph Receptors in Glioblastoma
    Author
    Sharma, P;Roberts, C;Herpai, D;Fokt, I;Priebe, W;Debinski, W;
    Year
    2020
    Journal
    Pharmaceuticals
カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください U.S.A.