IL-15 Protein, Human, Recombinant

IL-15 Protein, Human, Recombinant: 製品情報

純度
> 95 % as determined by SDS-PAGE.
内毒素
< 1 EU per μg of the protein as determined by the LAL method
活性
1. Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The EC50 for this effect is typically 2-8 ng/mL.
2. Labeled biotin to IL-15 Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of IL-15 Protein, Human, Recombinant, Biotinylated (Cat. 10360-HNCE) bound to Anti-IL15 Antibody was 0.1nM.
タンパク質の構築
A DNA sequence encoding the human IL15 (NP_000576.1) (Asn49-Ser162) was expressed.
NP No.
発現ホスト
E. coli
Human
予測N末端
Asn 49
分子量
The recombinant human IL15 consists of 114 amino acids and predicts a molecular mass of 12.8 kDa.
バッファー
Lyophilized from sterile 50 mM Tris, 200 mM NaCl, pH 8.0.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-15 Protein, Human, Recombinant: 画像

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells.The EC50 for this effect is typically 2-8 ng/mL.
Labeled biotin to IL-15 Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of IL-15 Protein, Human, Recombinant, Biotinylated (Cat. 10360-HNCE) bound to Anti-IL15 Antibody was 0.1nM.

IL-15 Protein, Human, Recombinant: 別名

IL-15 Protein, Human; Interleukin 15 Protein, Human

IL-15 背景情報

The protein encoded by the IL15 gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietic receptor subunits and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively, spliced transcript variants of this gene have been reported.
完全な名称
interleukin 15
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    Common G-Chain signaling pathway
参考文献
  • Mongini PKA, Gupta R, Boyle E, et al. TLR-9 and IL-15 synergy promotes the in vitro clonal expansion of chronic lymphocytic leukemia B cells. Journal of immunology (Baltimore, Md : 1950). 2015;195(3):901-923.
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