HER3/ERBB3 Protein, Human, Recombinant (His Tag), Biotinylated

HER3/ERBB3 Protein, Human, Recombinant (His Tag), Biotinylated: 製品情報

純度
> 95% as determined by SDS-PAGE.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
活性
1.Measured by its binding ability in a functional ELISA.
2.Immobilized biotinylated human ErbB3-His (Cat:10201-H08H-B) at 10μg/mL (100μL/well) can bind human Fc-NRG1(Cat:11609-H01H2), The EC50 of human Fc-NRG1 is 20- 60ng/mL.
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met1-Thr643) of human ErbB3 (NP_001973.2) precursor was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
NP No.
発現ホスト
HEK293 Cells
Human
予測N末端
Ser 20
分子量
The recombinant human ErbB3 protein consists of 635 amino and has a calculated molecular mass of 70.2 kDa.
バッファー
Lyophilized from sterile PBS,pH7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

HER3/ERBB3 Protein, Human, Recombinant (His Tag), Biotinylated: 画像

Measured by its binding ability in a functional ELISA. Immobilized biotinylated human ErbB3-His (Cat: 10201-H08H-B) at 10 μg/mL (100 μL/well) can bind human Fc-NRG1 (Cat: 11609-H01H2), The EC50 of human Fc-NRG1 is 20- 60 ng/mL.

HER3/ERBB3 Protein, Human, Recombinant (His Tag), Biotinylated: 別名

c-erbB-3 Protein, Human; c-erbB3 Protein, Human; EEBB3 Protein, Human; ErbB-3 Protein, Human; erbB3-S Protein, Human; HER3 Protein, Human; LCCS2 Protein, Human; MDA-BF-1 Protein, Human; p180-ErbB3 Protein, Human; p45-sErbB3 Protein, Human; p85-sErbB3 Protein, Human

HER3/ERBB3 背景情報

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
完全な名称
erb-b2 receptor tyrosine kinase 3
参考文献
  • Kraus M.H., et al.,(1989), Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc. Natl. Acad. Sci. U.S.A. 86:9193-9197.
  • Plowman G.D., et al., (1990), Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909.
  • Katoh M., et al.,(1993), c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.Biochem. Biophys. Res. Commun. 192:1189-1197.
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