CD73 Protein, Human, Recombinant (His Tag)

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CD73 Protein, Human, Recombinant (His Tag): 製品情報

純度
> 85 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by its ability to hydrolyze the 5’phosphate group from the substrate adenosine 5’monophosphate (AMP).
The specific activity is > 20,000 pmoles/min/μg.
タンパク質の構築
A DNA sequence encoding the human NT5E (NP_002517.1) (Met 1-Lys 547) without the propeptide was expressed, fused with a polyhistidine tag at the C-terminus.
NP No.
発現ホスト
HEK293 Cells
Human
予測N末端
Trp 27
分子量
The secreted recombinant human NT5E consists of 532 amino acids with the predicted molecular mass of 59.2 kDa.
バッファー
Lyophilized from sterile 20mM Tris, 120 mM NaCl, 4 mM CaCl2, 20 % glycerol, pH 7.5.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD73 Protein, Human, Recombinant (His Tag): 画像

CD73 Protein, Human, Recombinant (His Tag): 別名

CALJA Protein, Human; CD73 Protein, Human; E5NT Protein, Human; eN Protein, Human; eNT Protein, Human; NT Protein, Human; NT5 Protein, Human; NTE Protein, Human

CD73 背景情報

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.
完全な名称
5'-nucleotidase, ecto (CD73)
参考文献
  • Resta R. et al., 1997, Cell Signal. 9 (2): 131-9.
  • Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
  • Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
  • Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.
  • Adenosine Receptor Agonists And CD73 Effects On Avm Cell Calcification Markers
    Author
    Koliopoulos, T;
    Year
    2019
    Journal
    Thesis
    Application
    cell-based
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