CD40 Protein, Human, Recombinant (His Tag)

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CD40 Protein, Human, Recombinant (His Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by its binding ability in a functional ELISA
. Immobilized recombinant human CD40 at 2 μg/ml (100 μl/well) can bind biotinylated human CD40L with a linear range of 15.6-500 ng/ml.
タンパク質の構築
A DNA sequence encoding the human CD40 (NP_001241.1) extracellular domain (Met 1-Arg 193) was expressed, with a C-terminal polyhistidine tag.
NP No.
発現ホスト
HEK293 Cells
Human
予測N末端
Glu 21
分子量
The secreted recombinant human CD40 comprises 184 amino acids with a predicted molecular mass of 20.7 kDa. As a result of glycosylation, rhCD40 migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD40 Protein, Human, Recombinant (His Tag): 画像

Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD40 at 2 μg/ml (100 μl/well) can bind biotinylated human CD40L with a linear range of 15.6-500 ng/ml.

CD40 Protein, Human, Recombinant (His Tag): 別名

Bp50 Protein, Human; CDW40 Protein, Human; p50 Protein, Human; TNFRSF5 Protein, Human

CD40 背景情報

CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
完全な名称
CD40 molecule, TNF receptor superfamily member 5
研究分野
Related Pathways
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
参考文献
  • van Kooten C, et al. (2000). CD40-CD40 ligand. J Leukoc Biol. 67 (1): 2-17.
  • Bhushan A, et al. (2002). CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes. Immunol Res. 24 (3): 311-24.
  • Chatzigeorgiou A, et al. (2009) CD40/CD40L signaling and its implication in health and disease. Biofactors. 35(6): 474-83.
  • Li R, et al. (2009) Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance. Int J Mol Sci. 10(9): 3900-17.
  • Lievens D, et al. (2009) The multi-functionality of CD40L and its receptor CD40 in atherosclerosis. Thromb Haemost. 102(2): 206-14.
  • CD40 Enhances Type I Interferon Responses Downstream of CD47 Blockade, Bridging Innate and Adaptive Immunity
    Author
    de Silva, S;Fromm, G;Shuptrine, CW;Johannes, K;Patel, A;Yoo, KJ;Huang, K;Schreiber, TH;
    Year
    2019
    Journal
    Cancer Immunol Res
    Application
    ELISA ;SPR;luciferase reporter assay
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