CD32A Protein, Human, Recombinant (His & AVI Tag), Biotinylated

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CD32A Protein, Human, Recombinant (His & AVI Tag), Biotinylated: 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物素标记率
0.7-1 as determined by the HABA assay.
活性
1. Measured by its binding ability in a functional ELISA. Immobilized human IgG2 at 10 μg/ml (100 μl/well) can bind biotinylated human CD32 with a linear ranger of 0.16-0.8 μg/ml.
2. Using the Octet RED System, the affinity constant (Kd) of CD32a-AVI-His + BirA bound to human IgG was 12nM.
3. Using the Octet RED System, the affinity constant (Kd) of CD32A Protein, Human, Recombinant (His & AVI Tag), Biotinylated(Cat. 10374-H27H1-B) bound to Avastin was 0.03 μM.
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met 1-Met 210) of human CD32a (P12318-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. It is identical to FCGR2A131H/R in the reference.
NCBI Reference No.
発現ホスト
HEK293 Cells
Human
予測N末端
Gln 34
分子量
The recombinant human CD32aconsists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.6 KDa. It migrates as an approximately 33 KDa band due to glycosylation by SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD32A Protein, Human, Recombinant (His & AVI Tag), Biotinylated: 画像

Measured by its binding ability in a functional ELISA. Immobilized human IgG2 at 10 μg/ml (100 μl/well) can bind biotinylated human CD32 with a linear ranger of 0.16-0.8 μg/ml.
Using the Octet RED System, the affinity constant (Kd) of CD32A Protein, Human, Recombinant (His & AVI Tag), Biotinylated(Cat. 10374-H27H1-B) bound to Avastin was 0.03 μM.

CD32A Protein, Human, Recombinant (His & AVI Tag), Biotinylated: 別名

CD32 Protein, Human; CD32A Protein, Human; CDw32 Protein, Human; Fc gamma RIIA Protein, Human; FCG2 Protein, Human; FcGR Protein, Human; FCGR2 Protein, Human; FCGR2A1 Protein, Human; IGFR2 Protein, Human

CD32A 背景情報

完全な名称
Fc fragment of IgG receptor IIa
  • Enhanced Effector Functions Due to Antibody Defucosylation Depend on the Effector Cell Fcγ Receptor Profile
    Author
    Bruggeman, CW;Dekkers, G;Bentlage, AEH;Treffers, LW;Nagelkerke, SQ;Lissenberg-Thunnissen, S;Koeleman, CAM;Wuhrer, M;van den Berg, TK;Rispens, T;Vidarsson, G;Kuijpers, TW;
    Year
    2017
    Journal
    J. Immunol.
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