CD3 epsilon/CD3e Protein, Human, Recombinant (ECD, His Tag)

CD3 epsilon/CD3e Protein, Human, Recombinant (ECD, His Tag): 製品情報

純度
> 80 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Testing in progress
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met 1-Asp 126) of human CD3E (NP_000724.1) precursor was fused with a polyhistidine tag at the C-terminus.
NP No.
発現ホスト
HEK293 Cells
Human
予測N末端
Asp 23
分子量
The recombinant human CD3E consists of 115 amino acids after removal of the signal peptide and has a predicted molecular mass of 13.2 kDa. As a result of glycosylation, human CD3E migrates as an approximately 22.3 kDa band in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD3 epsilon/CD3e Protein, Human, Recombinant (ECD, His Tag): 画像

reducing conditions
Using the Octet RED System, the affinity constant (Kd) of CD3 epsilon / CD3e Protein, Human, Recombinant (His Tag) (Cat: 10977-H08H) bound to Human CD3 (humanized OKT3) Antibody-Human IgG4 was 3.8 nM.

CD3 epsilon/CD3e Protein, Human, Recombinant (ECD, His Tag): 別名

CD3 epsilon Protein, Human; IMD18 Protein, Human; T3E Protein, Human; TCRE Protein, Human

CD3 epsilon/CD3e 背景情報

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.
完全な名称
CD3e molecule, epsilon (CD3-TCR complex)
Related Pathways
  • T Cell Receptor Signaling Pathway
    T Cell Receptor Signaling Pathway
参考文献
  • Fischer A, et al. (2005) CD3 deficiencies. Curr Opin Allergy Clin Immunol. 5(6): 491-5.
  • Wang Y, et al. (2009) A conserved CXXC motif in CD3epsilon is critical for T cell development and TCR signaling. PLoS Biol. 7(12): e1000253.
  • Martnez-Martn N, et al. (2009) Cooperativity between T cell receptor complexes revealed by conformational mutants of CD3epsilon. Sci Signal. 2(83): ra43.
  • Deford-Watts LM, et al. (2009) The cytoplasmic tail of the T cell receptor CD3 epsilon subunit contains a phospholipid-binding motif that regulates T cell functions. J Immunol. 183(2): 1055-64.
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