CD155/PVR Protein, Human, Recombinant (ECD, hFc Tag)

CD155/PVR Protein, Human, Recombinant (ECD, hFc Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
1. Measured by its binding ability in a functional ELISA. Immobilized human CD226 His (Cat: 10565-H08H) at 2 μg/mL (100 μl/well) can bind human CD155 hFc (Cat: 10109-H02H), The EC50 of bind human CD155 hFc is 100-800 ng/mL.
2. Immobilized TIGIT Protein, Human, Recombinant (ECD, His Tag)(Cat:10917-H08H) at 2 μg/ml (100 μl/well) can bind CD155/PVR Protein, Human, Recombinant (ECD, hFc Tag)(Cat:10109-H02H), the EC50 of CD155/PVR Protein, Human, Recombinant (ECD, hFc Tag) is 25-140 ng/mL.
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met1-Asn343) of human CD155 (P15151-1) was expressed with the C-terminal fused Fc region of human IgG1.
Uniprot No.
発現ホスト
HEK293 Cells
Human
予測N末端
Trp 21
分子量
The recombinant human CD155/Fc is a disulfide-linked homodimer. The reduced monomer consists of 561 amino acids and predicts a molecular mass of 61.8 kDa. As a result of glycosylation, the rhCD155/Fc monomer migrates as approximately 95-105 kDa band in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD155/PVR Protein, Human, Recombinant (ECD, hFc Tag): 画像

Measured by its binding ability in a functional ELISA. Immobilized human CD226 His (Cat: 10565-H08H) at 2 μg/mL (100 μl/well) can bind human CD155 hFc (Cat: 10109-H02H), The EC50 of bind human CD155 hFc is 100-800 ng/mL.
Immobilized TIGIT Protein, Human, Recombinant (ECD, His Tag)(Cat:10917-H08H) at 2 μg/ml (100 μl/well) can bind CD155/PVR Protein, Human, Recombinant (ECD, hFc Tag)(Cat:10109-H02H), the EC50 of CD155/PVR Protein, Human, Recombinant (ECD, hFc Tag) is 25-140 ng/mL.

CD155/PVR Protein, Human, Recombinant (ECD, hFc Tag): 別名

CD155 Protein, Human; HVED Protein, Human; Necl-5 Protein, Human; NECL5 Protein, Human; PVS Protein, Human; TAGE4 Protein, Human

CD155/PVR 背景情報

CD155, commonly known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5), is a type I transmembrane single-span glycoprotein, and belongs to the nectins and nectin-like (Necl) subfamily. CD155 was originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV), an etiologic agent of the central nervous system disease poliomyelitis. The normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. CD155 may assist in an efficient humoral immune response generated within the intestinal immune system. It has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhesion, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.
完全な名称
poliovirus receptor
研究分野
参考文献
  • Freistadt MS, et al. (2000) Hematopoietic cells from CD155-transgenic mice express CD155 and support poliovirus replication ex vivo. Microb Pathog. 29(4): 203-12.
  • Sato T, et al. (2004) Involvement of heterophilic trans-interaction of Necl-5/Tage4/PVR/CD155 with nectin-3 in formation of nectin- and cadherin-based adherens junctions. Genes Cells. 9(9): 791-9.
  • Kakunaga S, et al. (2004) Enhancement of serum- and platelet-derived growth factor-induced cell proliferation by Necl-5/Tage4/poliovirus receptor/CD155 through the Ras-Raf-MEK-ERK signaling. J Biol Chem. 279(35): 36419-25.
  • Sato T, et al. (2005) Common signaling pathway is used by the trans-interaction of Necl-5/Tage4/PVR/CD155 and nectin, and of nectin and nectin during the formation of cell-cell adhesion. Cancer Sci. 96(9): 578-89.
  • Minami Y, et al. (2007) Involvement of up-regulated Necl-5/Tage4/PVR/CD155 in the loss of contact inhibition in transformed NIH3T3 cells. Biochem Biophys Res Commun. 352(4): 856-60.
  • CD226 Involves in Immune Synapse
    Author
    Wei, H;Sun, R;Tian, Z;Hou, S;Ge, K;Zheng, X;
    Year
    2014
    Journal
    J. Biol. Chem.
    Application
    binding
  • CD226 Protein Is Involved in Immune Synapse Formation and Triggers Natural Killer (NK) Cell Activation via Its First Extracellular Domain
    Author
    S Hou, K Ge, X Zheng, H Wei, R Sun, Z Tian
    Year
    2014
    Journal
    JOURNAL OF BIOLOGICAL CHEMISTRY
    Application
    binding
  • The receptors CD96 and CD226 oppose each other in the regulation of natural killer cell functions
    Author
    CJ Chan, L Martinet, S Gilfillan…
    Year
    2014
    Journal
    Nature immunology
  • TIGIT expression levels on CD4+ T cells are correlated with disease severity in patients with psoriasis
    Author
    Wang, FF;Wang, Y;Wang, L;Wang, TS;Bai, YP;
    Year
    2018
    Journal
    Clin. Exp. Dermatol.
    Application
    cell-based
  • TIGIT Can Exert Immunosuppressive Effects on CD8+ T Cells by the CD155/TIGIT Signaling Pathway for Hepatocellular Carcinoma In Vitro
    Author
    Zhang, C;Wang, Y;Xun, X;Wang, S;Xiang, X;Hu, S;Cheng, Q;Guo, J;Li, Z;Zhu, J;
    Year
    2020
    Journal
    J. Immunother.
    Application
    cell-based
カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください U.S.A.