CD32A Protein, Cynomolgus, Recombinant (His & AVI Tag)

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CD32A Protein, Cynomolgus, Recombinant (His & AVI Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
1. Immobilized Cynomolgus CD32a-AVI-His (Cat:90015-C27H) at 10 μg/ml (100 μl/well) can bind biotinylated Human IgG1, The EC50 of biotinylated Human IgG1 is 0.10-0.30 μg/ml.
2. Labeled biotin to CD32A Protein, Cynomolgus, Recombinant (His & AVI Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of CD32A Protein, Cynomolgus, Recombinant (His & AVI Tag), Biotinylated (Cat. 90015-C27H-B) bound to IgG1 Antibody was 6.3 nM.
タンパク質の構築
A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD32a (AAL92096.1) extracellular domain (Met 1-Gly 220) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.
GenBank No.
発現ホスト
HEK293 Cells
Cynomolgus
予測N末端
Ala 30
分子量
The recombinant cynomolgus CD32a consists of 206 amino acids and has a calculated molecular mass of 22.9 kDa. The apparent molecular mass of the recombinant protein is approximately 33-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
バッファー
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD32A Protein, Cynomolgus, Recombinant (His & AVI Tag): 画像

Labeled biotin to CD32A Protein, Cynomolgus, Recombinant (His & AVI Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of CD32A Protein, Cynomolgus, Recombinant (His & AVI Tag), Biotinylated (Cat. 90015-C27H-B) bound to IgG1 Antibody was 6.3 nM.

CD32A 背景情報

完全な名称
Fc fragment of IgG receptor IIa
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