Human cytomegalovirus (HCMV) Glycoprotein B / gB Protein (His Tag)

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Human cytomegalovirus (HCMV) Glycoprotein B / gB Protein (His Tag): 製品情報

純度
> 70 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Testing in progress
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907) of human CMV gB (AAA45920.1, with furin cleavage site mutated from 'RTKR' to 'TTQT') was fused with a polyhistidine tag at the C-terminus.
GenBank No.
発現ホスト
HEK293 Cells
CMV
予測N末端
Val 23
分子量
The recombinant human CMV glycoprotein B (transmembrane domain deletion) comprises 820 amino acids and has a predicted molecular mass of 93.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant CMV gB is approximately 130-140 kDa due to glycosylation.
バッファー
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Human cytomegalovirus (HCMV) Glycoprotein B / gB Protein (His Tag): 画像

Citation List

CMV Glycoprotein B 背景情報

Cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 97-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.
参考文献
  • Lopper M,et al. (2002). Disulfide bond configuration of human cytomegalovirus glycoprotein B. J Virol. 76(12): 6073-82.
  • Isaacson MK, et al. (2009) Human cytomegalovirus glycoprotein B is required for virus entry and cell-to-cell spread but not for virion attachment, assembly, or egress. J Virol. 83(8): 3891-903.
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