SARS-CoV-2 (2019-nCoV) NSP8-AVI Recombinant Protein

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SARS-CoV-2 (2019-nCoV) NSP8-AVI Recombinant Protein: 製品情報

純度
> 90 % as determined by SDS-PAGE.
内毒素
Please contact us for more information.
活性
Testing in progress
タンパク質の構築
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) NSP8 Protein (YP_009725304.1) (Ala1-Gln198) was expressed with a AVI tag at the C-terminus.
発現ホスト
E. coli
2019-nCoV
予測N末端
Gly
分子量
The recombinant SARS-CoV-2 (2019-nCoV) NSP8 consists of 220 amino acids and predicts a molecular mass of 24.17 kDa.
バッファー
Lyophilized from sterile 20mM Tris 500mM NaCl pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SARS-CoV-2 (2019-nCoV) NSP8-AVI Recombinant Protein: 画像

Coronavirus NSP8 背景情報

NSP8 is a nonstructural protein of coronavirus. NSP8 acts as a primase in RNA synthesis. NSP8 and NSP7 are essential co-factors of NSP12 (the catalytic subunit with RNA-dependent RNA polymerase activity) that can remarkably stimulates RdRp activity. The nsp12-nsp7-nsp8 subcomplex is defined as the minimal core component for mediating coronavirus RNA synthesis.
参考文献
  • Qi Peng,et al.Structural and Biochemical Characterization of the nsp12-nsp7-nsp8 Core Polymerase Complex from SARS-CoV-2.Cell Reports.2020
  • Yan Gao,et al.Structure of the RNA-dependent RNA polymerase from COVID-19 virus.Science.2020
  • Purnima Kumar,et al.The nonstructural protein 8 (nsp8) of the SARS coronavirus interacts with its ORF6 accessory protein.Virology.2007
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