SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (HPLC-verified)

COVID-19 Spike RBD Research.
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SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (HPLC-verified): 製品情報

純度
> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
活性
1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag)(40592-V05H), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) is 10-30 ng/mL.
2. Loaded Human ACE2 Protein (His tag) (Cat. No. 10108-H08H) on SA Biosensor, can bind SARS-CoV-2/2019-nCoV Spike/RBD Protein (RBD, mFc Tag) (Cat. No. 40592-V05H) with an affinity constant of 0.562 nM as determined by Octet RED System.
タンパク質の構築
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike Protein (RBD) (YP_009724390.1) (Arg319-Phe541) was expressed with the Fc region of mouse IgG1 at the C-terminus.
発現ホスト
HEK293 Cells
2019-nCoV
予測N末端
Arg319
分子量
The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) consists of 457 amino acids and predicts a molecular mass of 51.5 kDa.
バッファー
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (HPLC-verified): 画像

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag)(40592-V05H), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) is 15-50 ng/mL.
> 95 % as determined by SEC-HPLC.
Loaded Human ACE2 Protein (His tag) (Cat. No. 10108-H08H) on SA Biosensor, can bind SARS-CoV-2/2019-nCoV Spike/RBD Protein (RBD, mFc Tag) (Cat. No. 40592-V05H) with an affinity constant of 0.562 nM as determined by Octet RED System.
Loaded Human ACE2 Protein (His tag) (Cat. No. 10108-H08H) on SA Biosensor, can bind SARS-CoV-2/2019-nCoV Spike/RBD Protein (RBD, mFc Tag) (Cat. No. 40592-V05H) with an affinity constant of 0.562 nM as determined by Octet RED System.

SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (HPLC-verified): 別名

coronavirus spike Protein, 2019-nCoV; cov spike Protein, 2019-nCoV; ncov RBD Protein, 2019-nCoV; ncov s1 Protein, 2019-nCoV; ncov s2 Protein, 2019-nCoV; ncov spike Protein, 2019-nCoV; NCP-CoV RBD Protein, 2019-nCoV; NCP-CoV s1 Protein, 2019-nCoV; NCP-CoV s2 Protein, 2019-nCoV; NCP-CoV Spike Protein, 2019-nCoV; novel coronavirus RBD Protein, 2019-nCoV; novel coronavirus s1 Protein, 2019-nCoV; novel coronavirus s2 Protein, 2019-nCoV; novel coronavirus spike Protein, 2019-nCoV; RBD Protein, 2019-nCoV; S1 Protein, 2019-nCoV; S2 Protein, 2019-nCoV; Spike RBD Protein, 2019-nCoV

Coronavirus spike 背景情報

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
参考文献
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.

2019-nCoV Coronavirus spike タンパク質

  • Structural basis for the recognition of the 2019-nCoV by human ACE2
    Author
    Yan, R;Zhang, Y;Guo, Y;Xia, L;Zhou, Q;
    Year
    2020
    Journal
    bioRxiv
    Application
    binding
  • Analysis of SARS-CoV-2 Antibodies in COVID-19 Convalescent Blood using a Coronavirus Antigen Microarray
    Author
    De Assis, RR;Jain, A;Nakajima, R;Jasinskas, A;
    Year
    2020
    Journal
    BioRxiv
  • Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray
    Author
    Khan, S;Nakajima, R;Jain, A;De Assis, RR;Jasinskas, A;
    Year
    2020
    Journal
    BioRxiv
  • Discovery of Aptamers Targeting Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein
    Author
    Song, Y;Song, J;Wei, X;Huang, M;Sun, M;Zhu, L;Lin, B;Shen, H;Zhu, Z;Yang, C;
    Year
    2020
    Journal
    ChemRxiv
  • Human monoclonal antibodies block the binding of SARS-CoV-2 spike protein to angiotensin converting enzyme 2 receptor
    Author
    Ye, L;Chen, X;Li, R;Pan, Z;Qian, C;Yang, Y;You, R;
    Year
    2020
    Journal
    medRxiv
  • Humanized Single Domain Antibodies Neutralize SARS-CoV-2 by Targeting Spike Receptor Binding Domain
    Author
    Chi, X;Liu, X;Wang, C;Zhang, X;Ren, L;Jin, Q;Wang, J;
    Year
    2020
    Journal
    bioRxiv
  • Immunization with the receptor–binding domain of SARS-CoV-2 elicits antibodies cross-neutralizing SARS-CoV-2 and SARS-CoV without antibody-dependent enhancement
    Author
    Zang, J;Gu, C;Zhou, B;Zhang, C;Yang, Y;Xu, S;Zhang, X;
    Year
    2020
    Journal
    bioRxiv
    Application
    immunization
  • Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein
    Author
    Zost, SJ;Gilchuk, P;Chen, R;Case, JB;Reidy, JX;
    Year
    2020
    Journal
    bioRxiv
    Application
    FACS
  • Robust neutralization assay based on SARS-CoV-2 S-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressed BHK21 cells
    Author
    Xiong, H;Wu, Y;Cao, J;Yang, R;Ma, J;Qiao, X;Yao, X;
    Year
    2020
    Journal
    bioRxiv
    Application
    immunization
  • Structural Basis for Potent Neutralization of Betacoronaviruses by Single-domain Camelid Antibodies
    Author
    Wrapp, D;De Vlieger, D;Corbett, KS;Torres, GM;
    Year
    2020
    Journal
    BioRxiv
  • Structural basis for the recognition of the SARS-CoV-2 by full-length human ACE2
    Author
    Yan, R;Zhang, Y;Li, Y;Xia, L;Guo, Y;Zhou, Q;
    Year
    2020
    Journal
    Science
    Application
    stucture
  • Synthetic nanobodies targeting the SARS-CoV-2 receptor-binding domain
    Author
    Walter, JD;Hutter, CAJ;Zimmermann, I;Earp, J;Egloff, P;
    Year
    2020
    Journal
    BioRxiv
  • Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2
    Author
    Unknown, U;
    Year
    2020
    Journal
    science.sciencemag.org
    Application
    crystallization
  • Human H-ferritin presenting RBM of spike glycoprotein as potential vaccine of SARS-CoV-2
    Author
    Yao, D;Lao, F;Zhang, Z;Liu, Y;Cheng, J;Ding, F;Wang, X;Xi, L;Wang, C;Yan, X;Zhang, R;Ouyang, F;Ding, H;Ke, T;
    Year
    2020
    Journal
    bioRxiv
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