Human IFI30 qPCR Primer Pair

Human IFI30 qPCR Primer Pair: 一般情報

遺伝子情報
種:
Human
製品情報
オリゴヌクレオチドの種類:
qPCR Primers
成分:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCRプライマーの説明:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
アプリケーション & 品質管理
アプリケーション:
SYBR® Green-based quantitative real-time PCR (qPCR).
品質管理:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
保存 & 配送
配送方法:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
保存条件:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.
***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

特徴とメリット

独特なプライマー設計

ゲノムDNAの増幅を効果的に回避するために、このプライマーペアには少なくとも1つのプライマーまたは産物がイントロンを横切るように、特定の遺伝子の異なる変異体の保存領域でプライマーを設計します。

厳格なスクリーニング検証プロセス

プラスミド標準品でqPCRプライマーの感度、増幅効率、および特異性をスクリーニングし、陽性組織または細胞で検証し確認します。

均一なPCR条件、操作が簡単で、時間とコストを節約します

~100%という増幅効率で、RNA定量の正確さを保証

Human IFI30 qPCR Primer Pair: 別名

GILT qPCR Primer Pairs, Human; IFI-30 qPCR Primer Pairs, Human; IFI30 qPCR Primer Pairs, Human; IP-30 qPCR Primer Pairs, Human; IP30 qPCR Primer Pairs, Human

IFI30 背景情報

IFI30 belongs to the GILT family. This family includes the two characterized human gamma-interferon-inducible lysosomal thiol reductase (GILT) sequences: P13284 and Q9UL08. It also contains several other eukaryotic putative proteins with similarity to GILT. The aligned region contains three conserved cysteine residues. Besides, the two GILT sequences possess a C-X(2)-C motif that is shared by some of the other sequences in the family. This motif is thought to be associated with disulfide bond reduction. IFI30 is a lysosomal thiol reductase that can reduce protein disulfide bonds. It facilitates the generation of MHC class II-restricted epitopes from disulfide bond-containing antigens by the endocytic reduction of disulfide bonds. It also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or cross-presentation. IFI30 may facilitate the complete unfolding of proteins destined for lysosomal degradation and plays an important role in antigen processing.
完全な名称
interferon gamma inducible protein 30
参考文献
  • Haque MA, et al. (2002) Absence of gamma-Interferon-inducible Lysosomal Thiol Reductase in Melanomas Disrupts T Cell Recognition of Select Immunodominant Epitopes. J Exp Med. 195(10):1267-77.
  • Phan UT, et al. (2000) Gamma-interferon-inducible lysosomal thiol reductase (GILT). Maturation, activity, and mechanism of action. J Biol Chem. 275(34):25907-14.
  • Phan UT, et al. (2001) Multiple species express thiol oxidoreductases related to GILT. Immunogenetics. 53(4):342-6.
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